Expression analysis and single-nucleotide polymorphisms of SYNDIG1L and UNC13C genes associated with thoracic vertebral numbers in sheep (Ovis aries)

The objective of the current study was to analyze expression levels of synapse differentiation inducing 1-like (SYNDIG1L) and unc-13 homolog C (UNC13C) genes in different tissues, while single-nucleotide polymorphisms (SNPs) of two genes were associated with multiple thoracic vertebrae traits in bot...

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Bibliographic Details
Published in:Archiv für Tierzucht 2021-04, Vol.64 (1), p.131-138
Main Authors: Zhong, Ying-Jie, Yang, Yang, Wang, Xiang-Yu, Di, Ran, Chu, Ming-Xing, Liu, Qiu-Yue
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose tissues
Adrenal glands
Analysis
Association analysis
Brain
Breeding of animals
Cerebellum
Gene expression
Gene polymorphism
Genes
Genetic aspects
genetic markers
Genetic research
Genomes
genotyping
heart
Hogs
Homology
kidneys
Laboratories
liver
longissimus muscle
lungs
Muscles
Mutation
Nucleotides
Original Study
Ovis aries
Polymerase chain reaction
Polymorphism
quantitative polymerase chain reaction
Sheep
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Spleen
Statistics
synapse
Thorax
Uterus
Vertebrae
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Summary:The objective of the current study was to analyze expression levels of synapse differentiation inducing 1-like (SYNDIG1L) and unc-13 homolog C (UNC13C) genes in different tissues, while single-nucleotide polymorphisms (SNPs) of two genes were associated with multiple thoracic vertebrae traits in both Small-tailed Han sheep (STH) and Sunite sheep (SNT). The expression levels of SYNDIG1L and UNC13C were analyzed in the brain, cerebellum, heart, liver, spleen, lung, kidney, adrenal gland, uterine horn, longissimus muscle, and abdominal adipose tissues of two sheep breeds with different thoracic vertebral number (TVN) sheep (T13 groups and T14 groups) by real-time quantitative polymerase chain reaction (RT-qPCR). Meanwhile, the polymorphisms of UNC13C gene g.52919279C>T and SYNDIG1L gene g.82573325C>A in T14 and T13 were genotyped by the Sequenom MassARRAY® SNP assay, and association analysis was performed with the TVN. The results demonstrated that UNC13C gene was extensively expressed in 11 tissues. The expression of UNC13C gene in longissimus muscle of T14 groups of STH was significantly higher than that of T13 groups (P
ISSN:2363-9822
0003-9438
2363-9822
DOI:10.5194/aab-64-131-2021