Experimental Parameters Influence the Observed Antimicrobial Response of Oak Wood ( Quercus petraea )

The present investigation aimed to utilize a direct wood disc diffusion method to study the influence of plane of cutting, cutting method, sterilization method, and origin of tree on the antimicrobial activity of wood material. Six oak wood trees ( ) were collected from 3 different locations in Fran...

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Bibliographic Details
Published in:Antibiotics (Basel) 2020-08, Vol.9 (9), p.535-8
Main Authors: Munir, Muhammad Tanveer, Pailhories, Hélène, Eveillard, Matthieu, Irle, Mark, Aviat, Florence, Federighi, Michel, Belloncle, Christophe
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
agar diffusion
Agricultural sciences
Antibiotics
Antimicrobial activity
Antimicrobial agents
Autoclaving
Bacteria
Cuttings
Diffusion
Environmental Sciences
Food
Gamma irradiation
Gram-positive bacteria
Irradiation
Lasers
Life Sciences
Methods
Moisture content
Nosocomial infections
Quercus petraea
Staphylococcus aureus
Statistical analysis
Sterilization
Trees
Variance analysis
Wood
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Summary:The present investigation aimed to utilize a direct wood disc diffusion method to study the influence of plane of cutting, cutting method, sterilization method, and origin of tree on the antimicrobial activity of wood material. Six oak wood trees ( ) were collected from 3 different locations in France. They were cut into 4 mm thick slices with either transverse (RT), tangential (LT) or radial (LR) faces. Round discs (diameter 9.95 ± 0.1 mm) were cut from the slices via a laser machine or a manual punch machine, and were sterilized with gamma irradiation (25 kGy) or autoclaving (121 °C). The antimicrobial activity of wood was tested using a direct diffusion method against and isolates. The zone of inhibition around the wooden disc was recorded following the recommendations used for antibiotics tests. The results showed that was more susceptible than , to the chemicals that diffused from the wood. The transverse face discs exhibited higher antimicrobial activity. Samples that had been sterilized by autoclaving showed significantly ( < 0.05) lower antimicrobial activity, whereas the cutting method and origin of tree did not influence the antimicrobial activity of wood material. Therefore, the choice of sterilization method and cutting planes must be taken into account while studying and interpreting the antibacterial properties of wood material.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9090535