Novel exc Genes Involved in Formation of the Tubular Excretory Canals of Caenorhabditis elegans

Abstract Regulation of luminal diameter is critical to the function of small single-celled tubes, of which the seamless tubular excretory canals of Caenorhabditis elegans provide a tractable genetic model. Mutations in several sets of genes exhibit the Exc phenotype, in which canal luminal growth is...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2019-05, Vol.9 (5), p.1339-1353
Main Authors: Al-Hashimi, Hikmat, Chiarelli, Travis, Lundquist, Erik A, Buechner, Matthew
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans - ultrastructure
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
Charcot-Marie-Tooth Syndrome Type 4H
endosomes
EXC-5
Exocrine Glands - metabolism
Exocrine Glands - ultrastructure
Gene Knockdown Techniques
Genetic Association Studies
Genotype
lumen formation
Mutant Screen Report
Mutation
Phenotype
RNA Interference
Tubulogenesis
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Summary:Abstract Regulation of luminal diameter is critical to the function of small single-celled tubes, of which the seamless tubular excretory canals of Caenorhabditis elegans provide a tractable genetic model. Mutations in several sets of genes exhibit the Exc phenotype, in which canal luminal growth is visibly altered. Here, a focused reverse genomic screen of genes highly expressed in the canals found 18 genes that significantly affect luminal outgrowth or diameter. These genes encode novel proteins as well as highly conserved proteins involved in processes including gene expression, cytoskeletal regulation, and vesicular and transmembrane transport. In addition, two genes act as suppressors on a pathway of conserved genes whose products mediate vesicle movement from early to recycling endosomes. The results provide new tools for understanding the integration of cytoplasmic structure and physiology in forming and maintaining the narrow diameter of single-cell tubules.
ISSN:2160-1836
2160-1836
DOI:10.1534/g3.119.200626