Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improv...

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Bibliographic Details
Published in:Communications biology 2023-12, Vol.6 (1), p.1270-1270, Article 1270
Main Authors: Ito, Akira, Matsuda, Nagisa, Ukita, Yumiko, Okumura, Misako, Chihara, Takahiro
Format: Article
Language:English
Subjects:
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Animals
Bioluminescence
Biomedical and Life Sciences
Drosophila
Drosophila - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Fusion protein
Gene Expression
Innate immunity
Insects
Life Sciences
Luciferases - genetics
Luciferases - metabolism
Luciferins
Luminescent Measurements - methods
Oral administration
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Summary:Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improved signal detection. We evaluate the potential of Akaluc/AkaLumine in Drosophila melanogaster to establish a highly sensitive, non-invasive, and temporal detection method for gene expression. Our results show that oral administration of AkaLumine to flies expressing Akaluc provided a higher luminescence signal than Luc/D-luciferin, with no observed harmful effects on flies. The Akaluc/AkaLumine system allows for monitoring of dynamic temporal changes in gene expression. Additionally, using the Akaluc fusion gene allows for mRNA splicing monitoring. Our findings indicate that the Akaluc/AkaLumine system is a powerful bioluminescence tool for analyzing gene expression in deep tissues and small numbers of cells in Drosophila . In Drosophila melanogaster , the red-shifted bioluminescence system Akaluc/AkaLumine is useful for studying gene expression, cell numbers, and innate immunity levels in deep tissues without harming the individual.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-023-05628-x