Insights into polyethylene biodegradative fingerprint of Pseudomonas citronellolis E5 and Rhodococcus erythropolis D4 by phenotypic and genome-based comparative analyses

• Published in: Frontiers in bioengineering and biotechnology 2024-12, Vol.12, p.1472309
• Main Authors: Zampolli, Jessica, Collina, Elena, Lasagni, Marina, Di Gennaro, Patrizia
• Format: Article
• Language: English
• Subjects: Bioengineering and Biotechnology; gene clusters; genome analysis; laccase activity; polyethylene biodegradation; Pseudomonas citronellolis; Rhodococcus erythropolis
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2024.1472309

Polyethylene (PE) is the most-produced polyolefin, and consequently, it is the most widely found plastic waste worldwide. PE biodegradation is under study by applying different (micro)organisms in order to understand the biodegradative mechanism in the majority of microbes. This study aims to identify novel bacterial species with compelling metabolic potential and strategic genetic repertoires for PE biodegradation. E5 is newly isolated from solid organic waste contaminated with plastic debris, and D4 was selected for its promising potential in biodegradable plastic determined by its genetic repertoire. E5 was selected for its ability to grow on PE as the only carbon and energy source. Meaningful extracellular secreted laccase activity was also characterized for D4 during growth on PE (E5 and D4 strains have a laccase activity of (2 ± 1)×10  U mg and (3 ± 1)×10  U mg , respectively). Despite the highest level of cell numbers recorded at 7 days of growth on PE for both strains, the patterns of the metabolic products obtained and degraded during 60 days on PE were dissimilar in the two bacteria at different sampling times. However, they mainly produced metabolites belonging to carboxylic acids and alkanes with varying numbers of carbons in the aliphatic chains. Whole-genome sequence analyses of E5 compared to . D4 and genetic determinant prediction (by gene annotation and multiple sequence alignment with reference gene products) have been performed, providing a list of 16 and 42 gene products putatively related to different metabolic steps of PE biodegradation. Altogether, these results support insights into PE biodegradation by bacteria of the and genera from metabolic and genetic perspectives as a base to build up novel biotechnological platforms.