Emerging MDR-Mycobacterium avium subsp. avium in house-reared domestic birds as the first report in Egypt

• Published in: BMC microbiology 2021-08, Vol.21 (1), p.237-237, Article 237
• Main Authors: Algammal, Abdelazeem M, Hashem, Hany R, Al-Otaibi, Amenah S, Alfifi, Khyreyah J, El-Dawody, Esraa M, Mahrous, Eman, Hetta, Helal F, El-Kholy, Ali W, Ramadan, Hazem, El-Tarabili, Reham M
• Format: Article
• Language: English
• Subjects: Acids; Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiogram; Antibiotic resistance; Antibiotics; Antimicrobial agents; Aquatic birds; Azithromycin; Bacterial Zoonoses - epidemiology; Birds; Birds - microbiology; Cage birds; Clofazimine; Diagnosis; Diarrhea; Doxycycline; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Multiple, Bacterial; Ducks - microbiology; Egypt - epidemiology; Emerging communicable diseases; Endangered & extinct species; Feces; Feces - microbiology; Food contamination & poisoning; Geese - microbiology; Genes; Genetic aspects; Health aspects; Health care; Health risks; House-reared domestic birds, prevalence; Isoniazid; M. avium subsp. avium; MDR, antibiotic resistance-related genes; Microbial Sensitivity Tests; Multidrug resistance; Mycobacterium - drug effects; Mycobacterium - genetics; Mycobacterium - isolation & purification; Mycobacterium avium; Mycobacterium Infections - epidemiology; Mycobacterium Infections - veterinary; Oxytetracycline; Pathogens; Pets - microbiology; Public health; Rifampin; Risk factors; RpoB protein; Streptomycin; Tuberculosis; Zoonoses
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-021-02287-y

Avian tuberculosis is a chronic and zoonotic disease that affects a wide variety of birds, mammals, and humans. This study aimed to estimate the frequency of Mycobacterium avium subsp. avium in some domestic birds based on molecular diagnosis, antibiogram profile, and PCR-based detection of inhA, rpoB, rpsL, and otrB antibiotic resistance-related genes. A total of 120 fecal samples were collected from small flocks of house-reared domestic birds at Ismailia Governorate, Egypt. The collected samples were processed and subjected to the bacteriological examination. The antimicrobial susceptibility testing of the recovered isolates was performed using the broth microdilution method for the detection of minimum inhibitory concentrations (MICs). The genetic detection of the IS901confirmatory gene, inhA, rpoB, rpsL, and otrB genes was carried out using PCR. The frequency of M. avium subsp. avium was 4.1% (5/120); 10% (4/40) in ducks, and 2.5% (1/10) in geese. The identification of the recovered isolates was confirmed using PCR, where all the tested isolates were positive for IS901confirmatory gene. The results of the broth microdilution method revealed that most of the recovered isolates exhibited multidrug resistance (MDR) to isoniazid, rifampicin, streptomycin, oxytetracycline, and doxycycline, and harbored the inhA, rpoB, rpsL, and otrB genes. In brief, to the best of our knowledge this is the first report that emphasized the emergence of avian tuberculosis in house-reared domestic birds in Egypt. The emergence of MDR- M. avium subsp. avium is considered a public health threat. Emerging MDR-M. avium subsp. avium in domestic birds are commonly harbored the IS901, inhA, rpoB, rpsL, and otrB genes. Azithromycin and clofazimine revealed a promising in-vitro antibacterial activity against M. avium subsp. avium.