3D printed elastic hydrogel conduits with 7,8-dihydroxyflavone release for peripheral nerve repair

Nerve guide conduit is a promising treatment for long gap peripheral nerve injuries, yet its efficacy is limited. Drug-releasable scaffolds may provide reliable platforms to build a regenerative microenvironment for nerve recovery. In this study, an elastic hydrogel conduit encapsulating with prodru...

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Bibliographic Details
Published in:Materials today bio 2023-06, Vol.20, p.100652-100652, Article 100652
Main Authors: Wu, Wenbi, Dong, Yinchu, Liu, Haofan, Jiang, Xuebing, Yang, Ling, Luo, Jing, Hu, Yu, Gou, Maling
Format: Article
Language:English
Subjects:
3D printing
Biomaterials
Drug delivery
Full Length
Nerve conduit
Peripheral nerve repair
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Summary:Nerve guide conduit is a promising treatment for long gap peripheral nerve injuries, yet its efficacy is limited. Drug-releasable scaffolds may provide reliable platforms to build a regenerative microenvironment for nerve recovery. In this study, an elastic hydrogel conduit encapsulating with prodrug nanoassemblies is fabricated by a continuous 3D printing technique for promoting nerve regeneration. The bioactive hydrogel is comprised of gelatin methacryloyl (GelMA) and silk fibroin glycidyl methacrylate (SF-MA), exhibiting positive effects on adhesion, proliferation, and migration of Schwann cells. Meanwhile, 7,8-dihydroxyflavone (7,8-DHF) prodrug nanoassemblies with high drug-loading capacities are developed through self-assembly of the lipophilic prodrug and loaded into the GelMA/SF-MA hydrogel. The drug loading conduit could sustainedly release 7,8-DHF to facilitate neurite elongation. A 12 ​mm nerve defect model is established for therapeutic efficiency evaluation by implanting the conduit through surgical suturing with rat sciatic nerve. The electrophysiological, morphological, and histological assessments indicate that this conduit can promote axon regeneration, remyelination, and function recovery by providing a favorable microenvironment. These findings implicate that the GelMA/SF-MA conduit with 7,8-DHF release has potentials in the treatment of long-gap peripheral nerve injury. 3D printed elastic GelMA/SF-MA hydrogel conduits with 7,8-DHF prodrug nanoassemblies for peripheral nerve repair. [Display omitted]
ISSN:2590-0064
2590-0064
DOI:10.1016/j.mtbio.2023.100652