Evaluation of Traumatic Spinal Cord Injury in a Rat Model Using 99mTc-GA-5 as a Potential In Vivo Tracer

Spinal cord injury (SCI) refers to the damage suffered in the spinal cord by any trauma or pathology. The purpose of this work was to determine whether 99mTc-GA-5, a radiotracer targeting Glial Fibrillary Acidic Protein (GFAP), can reveal in vivo the reactivation of astrocytes in a murine model with...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-11, Vol.26 (23), p.7138
Main Authors: Izquierdo-Sánchez, Vanessa, Zambrano-Rodríguez, Pablo C., Peña-Merino, Nadia, Bolaños-Puchet, Sirio, Reyes-Alva, Horacio J., Martínez-Cruz, Angelina, Muñiz-Hernández, Saé, Guízar-Sahagún, Gabriel, Medina, Luis Alberto
Format: Article
Language:English
Subjects:
99mTc-GA-5
Animal models
Antibodies
Astrocytes
astrogliosis
Biodistribution
Biomarkers
Blood-brain barrier
Computed tomography
GA-5 monoclonal antibody
GFAP
Glial fibrillary acidic protein
In vivo methods and tests
Ischemia
Ligands
microSPECT/CT imaging
Molecular weight
Monoclonal antibodies
Physiology
Radioactive tracers
Radiochemistry
Scars
Single photon emission computed tomography
Spinal cord injuries
spinal cord injury
Trauma
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Summary:Spinal cord injury (SCI) refers to the damage suffered in the spinal cord by any trauma or pathology. The purpose of this work was to determine whether 99mTc-GA-5, a radiotracer targeting Glial Fibrillary Acidic Protein (GFAP), can reveal in vivo the reactivation of astrocytes in a murine model with SCI. A method for the 99mTc radiolabeling of the mouse anti-GFAP monoclonal antibody GA-5 was implemented. Radiochemical characterization was performed, and radioimmunohistochemistry assays were used to evaluate the integrity of 99mTc-GA-5. MicroSPECT/CT was used for in vivo imaging to trace SCI in the rats. No alterations in the GA-5’s recognition/specificity ability were observed after the radiolabeling. The GA-5’s radiolabeling procedure implemented in this work offers a practical method to allow the in vivo following of this monoclonal antibody to evaluate its biodistribution and specificity for GFAP receptors using SPECT/CT molecular imaging.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26237138