Phytochemical Profile, Antioxidant Potential, Proximate and Trace Elements Composition of Leaves, Stems and Ashes from 12 Combretum spp. Used as Food Additives

• Published in: International Journal of Plant Biology 2022-11, Vol.13 (4), p.561-578
• Main Authors: Mathipa, Morongwa Mary, Mphosi, Maboko Samuel, Masoko, Peter
• Format: Article
• Language: English
• Subjects: antioxidants and phytochemicals; Combretum; proximate
• ISSN: 2037-0164; 2037-0164
• DOI: 10.3390/ijpb13040045

In this study, twelve Combretum spp. were investigated for their phytochemical content, antioxidant potential, and their proximate and trace elements/minerals composition. The qualitative phytochemical composition of the leaves, stems, and ashes of Combretum plants analysed revealed the presence of saponins, tannins, terpenoids, steroids, cardiac glycosides, and flavonoids. The following phytoconstituents were lost in the ashes; tannins apart from C. mkuzense and C. padoides; cardiac glycosides; and flavonoids. The quantitative phytochemical analyses revealed that both the leaves, stems, and some ashes such as C. apiculatum and C. vendae contained levels of phenolic compounds, tannins, and flavonoids. DPPH screening method indicated great scavenging activity with the 70% acetone leaf extracts of C. kraussii, C. zeyheriim, and C. mkuzense. There was a significant decrease in the antioxidant activity in the ashes compared to the leaves and the stems. AOAC and ICPE protocols performed the proximate and nutritional analysis of the 70% acetone extracts. The extracts had substantial amounts of ash, moisture, protein, and energy. The leaves and ashes of C. adenogonium and C. apiculatum could provide a good source of calcium in the diet. This study presents valuable information on the phytochemical composition, nutritional composition, and antioxidant properties of some Combretum species.