Association of Tumor Necrosis Factor Alpha 308 G/A Polymorphism with Asthma in Pakistani Population

• Published in: Iranian journal of allergy, asthma, and immunology asthma, and immunology, 2015-06, Vol.14 (3), p.287-291
• Main Authors: Saba, Nusrat, Yusuf, Osman, Rehman, Sadia, Munir, Saeeda, Bashir, Naghman, Mansoor, Atika, Raja Kaukab, Ghazala
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Asthma; Asthma - genetics; Child; Confidence intervals; Cytokines; Deoxyribonucleic acid; Disease; DNA; DNA polymerase; Ethnicity; Female; Gangrene; Genes; Genetic Predisposition to Disease; Genetic testing; Genotype; Genotype & phenotype; Humans; Male; Middle Aged; Pakistan; Pakistani population; Polymorphism, Single Nucleotide; Studies; TNF alpha 308; Tumor Necrosis Factor-alpha - genetics
• ISSN: 1735-1502; 1735-5249

Asthma is a chronic inflammatory and remodeling disorder of the airways, in which many cells, cellular elements, and cytokines play important roles. The role of tumor necrosis factor-α (TNF-α) in asthma is unclear in Pakistani population. The aim of this study was to assess the relationship between TNF-α-308 polymorphism and asthma. Polymorphism of TNF-α (G-308-A locus; rs 1800629) in 329 asthmatic patients and 151 healthy controls was evaluated. DNA was prepared from blood samples of cases and controls. Samples were genotyped for TNF-α 308 G/A polymorphism. There was no significant difference in the frequency of GG (OR 1.049 with 95% CI 0.68-1.63) and GA (OR 0.987 with 95% CI 0.64-1.53) genotypes of TNF-α-308. The AA genotype was absent in cases and only one AA genotype was observed in the controls. The genetic polymorphism of TNF-α does not seem to be associated with asthma in Pakistani population.