Characterization of protein arginine methyltransferase of TgPRMT5 in Toxoplasma gondii

• Published in: Parasites & vectors 2019-05, Vol.12 (1), p.221-6, Article 221
• Main Authors: Liu, Min, Li, Fen-Xiang, Li, Chun-Yuan, Li, Xiao-Cong, Chen, Long-Fei, Wu, Kun, Yang, Pei-Liang, Lai, Zhi-Fa, Liu, Ting-Kai, Sullivan, Jr, William J, Cui, Liwang, Chen, Xiao-Guang
• Format: Article
• Language: English
• Subjects: Amino acids; Antigenic determinants; Arginine; Bradyzoites; Cell division; Chromatin; Cloning; Cytoplasm; Cytoplasm - chemistry; Deoxyribonucleic acid; DNA; DNA damage; DNA methylation; DNA repair; Enzymes; Epigenesis, Genetic; Epigenetic inheritance; Epigenetics; Epitopes; Eukaryotes; Gene expression; Gene regulation; Genetic aspects; Hemagglutinins; Histone; Identification and classification; Infections; Lectins; Life Cycle Stages; Localization; Lymphoma; Medical schools; Metabolism; Methylation; Methyltransferases; Nucleic acids; Nucleotide sequence; Parasites; Parasitological research; Physiological aspects; Post-translation; Post-translational modifications; Protein arginine methyltransferase; Protein-Arginine N-Methyltransferases - genetics; Protein-Arginine N-Methyltransferases - metabolism; Proteins; Protozoa; Pyrimethamine; Ribonucleic acid; RNA; Short Report; Stem cells; Tachyzoites; Toxoplasma; Toxoplasma - enzymology; Toxoplasma - genetics; Toxoplasma gondii; Transcription; Transcription (Genetics); Transferases
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-019-3464-1

Protein arginine methylation is a prevalent post-translational modification. The protein arginine methyltransferase family (PRMT) is involved in many cellular processes in eukaryotes, including transcriptional regulation, epigenetic regulation, RNA metabolism, and DNA damage repair. Toxoplasma gondii, an opportunistic protozoan parasite, encodes five conserved PRMTs. PRMT5 is thought to be responsible for substantial PRMT activity in T. gondii; however, it has not yet been characterized. We tagged the 3' end of the endogenous TgPRMT5 genomic locus with sequence encoding a 3X hemagglutinin (HA) epitope. IFA and WB were performed to check the expression and subcellular localization of TgPRMT5 in tachyzoites and bradyzoites. In vitro methylation assays were performed to determine whether endogenous TgPRMT5 has arginine methyltransferase activity. IFA and WB results showed that T. gondii PRMT5 (TgPRMT5) was localized in the cytoplasm in the tachyzoite stage; however, it shifts largely to the nuclear compartment in the bradyzoite stage. The in vitro methylation showed that TgPRMT5 has authentic type II PRMT activity and forms monomethylarginines and symmetric dimethylarginines. We determined the expression and cellular localization of TgPRMT5 in tachyzoites and bradyzoites and confirmed its type II PRMT activity. We demonstrated the major changes in expression and cellular localization of TgPRMT5 during the tachyzoite and bradyzoite stages in T. gondii. Our findings suggest that TgPRMT5 protein may be involved in tachyzoite-bradyzoite transformation.