Biochemical Characterisation of Sis: A Distinct Thermophilic PETase with Enhanced NanoPET Substrate Hydrolysis and Thermal Stability

Polyethylene terephthalate (PET) degradation by enzymatic hydrolysis is significant for addressing plastic pollution and fostering sustainable waste management practices. Identifying thermophilic and thermostable PET hydrolases is particularly crucial for industrial bioprocesses, where elevated temp...

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Published in:International journal of molecular sciences 2024-08, Vol.25 (15), p.8120
Main Authors: Ercolano, Carmen, Iacono, Roberta, Cafaro, Valeria, Pizzo, Elio, Giovannelli, Donato, Feuerriegel, Golo, Streit, Wolfgang R, Strazzulli, Andrea, Moracci, Marco
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Chemical bonds
Environmental impact
enzyme discovery
Enzyme Stability
Enzymes
Hydrolases
Hydrolases - chemistry
Hydrolases - metabolism
Hydrolysis
Kinetics
PET hydrolysis
Phylogeny
Polyethylene terephthalate
Polyethylene Terephthalates - chemistry
Polyethylene Terephthalates - metabolism
Substrate Specificity
Temperature
thermozymes
Waste management
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container_end_page
container_issue 15
container_start_page 8120
container_title International journal of molecular sciences
container_volume 25
creator Ercolano, Carmen
Iacono, Roberta
Cafaro, Valeria
Pizzo, Elio
Giovannelli, Donato
Feuerriegel, Golo
Streit, Wolfgang R
Strazzulli, Andrea
Moracci, Marco
description Polyethylene terephthalate (PET) degradation by enzymatic hydrolysis is significant for addressing plastic pollution and fostering sustainable waste management practices. Identifying thermophilic and thermostable PET hydrolases is particularly crucial for industrial bioprocesses, where elevated temperatures may enhance enzymatic efficiency and process kinetics. In this study, we present the discovery of a novel thermophilic and thermostable PETase enzyme named Sis, obtained through metagenomic sequence-based analysis. Sis exhibits robust activity on nanoPET substrates, demonstrating effectiveness at temperatures up to 70 °C and displaying exceptional thermal stability with a melting temperature (T ) of 82 °C. Phylogenetically distinct from previously characterised PET hydrolases, Sis represents a valuable addition to the repertoire of enzymes suitable for PET degradation.
doi_str_mv 10.3390/ijms25158120
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subjects Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Chemical bonds
Environmental impact
enzyme discovery
Enzyme Stability
Enzymes
Hydrolases
Hydrolases - chemistry
Hydrolases - metabolism
Hydrolysis
Kinetics
PET hydrolysis
Phylogeny
Polyethylene terephthalate
Polyethylene Terephthalates - chemistry
Polyethylene Terephthalates - metabolism
Substrate Specificity
Temperature
thermozymes
Waste management
title Biochemical Characterisation of Sis: A Distinct Thermophilic PETase with Enhanced NanoPET Substrate Hydrolysis and Thermal Stability
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