Comparison of tumor cell numbers and 22C3 PD-L1 expression between cryobiopsy and transbronchial biopsy with endobronchial ultrasonography-guide sheath for lung cancer

• Published in: Respiratory research 2019-08, Vol.20 (1), p.185-185, Article 185
• Main Authors: Arimura, Ken, Kondo, Mitsuko, Nagashima, Yoji, Kanzaki, Masato, Kobayashi, Fumi, Takeyama, Kiyoshi, Tamaoki, Jun, Tagaya, Etsuko
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; B7-H1 Antigen - biosynthesis; B7-H1 Antigen - genetics; Biopsy - methods; Bronchi - diagnostic imaging; Bronchi - metabolism; Bronchi - pathology; Care and treatment; Cell Count - methods; Cellular proteins; Cryobiopsy; Cryosurgery - methods; Development and progression; Endobronchial ultrasonography-guide sheath; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Male; Middle Aged; Peripheral pulmonary lesions; Programmed death ligand 1 expression; Prospective Studies; The number of tumor cells; Ultrasonography, Interventional - methods
• ISSN: 1465-993X; 1465-9921; 1465-993X
• DOI: 10.1186/s12931-019-1162-3

We previously reported cryobiopsy (Cryo) with endobronchial ultrasonography-guide sheath (EBUS-GS) for peripheral pulmonary lesions (PPLs) provides significantly larger tissues than transbronchial biopsy (TBB) and provides high quantity and quality DNA for gene analysis by next generation sequencing. However, the tumor cell yields and programmed death ligand 1 (PD-L1) expression between each approach have not been compared. Here, we assessed the tumor cell numbers and PD-L1 expression for Cryo with EBUS-GS for PPLs and TBB in patients with lung cancer. Sixteen patients were enrolled in this prospective study from June to November 2017 at Tokyo Women's Medical University Hospital. The number of tumor cells from a single biopsy, total number of tumor cells, average number of tumor cells, and 22C3 PD-L1 expression (≥ 50% and ≥ 1%) were compared between Cryo and TBB. The numbers of tumor cells from a single biopsy, total numbers of tumor cells, and average numbers of tumor cells obtained by Cryo were significantly larger than those obtained by TBB (Cryo [means ± standard errors of the means]: 1321 ± 303.7, 1981 ± 411.7, and 1406 ± 310.3; TBB: 208.8 ± 38.24, 1044 ± 189.0, and 208.8 ± 37.81; P