Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance in Lentinula edodes

• Published in: Frontiers in microbiology 2022-06, Vol.13, p.910255-910255
• Main Authors: Xu, Ruiping, Zhou, Shasha, Song, Jiaxin, Zhong, Haiying, Zhu, Tianwen, Gong, Yuhua, Zhou, Yan, Bian, Yinbing
• Format: Article
• Language: English
• Subjects: 2,4-dichlorophenoxyacetic acid; comparative transcriptome; fatty acid metabolism; heat stress; Lentinula edodes; Microbiology
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.910255

As the widest cultivated edible mushroom worldwide, Lentinula edodes suffers serious yield and quality losses from heat stress during growth and development, and in our previous study, exogenous 2,4-Dichlorophenoxyacetic acid (2,4-D) was found to improve the thermotolerance of L. edodes strain YS3357, but the molecular mechanism remains unclear. Here, we explored the potential protective mechanism of exogenous 2,4-D against heat stress by transcriptome analysis. 2,4-D possible improve the thermotolerance of L. edodes through regulating antioxidant genes, transcription factors, energy-provision system, membrane fluidity, and cell wall remodeling. Furthermore, 2,4-D was also found to regulate the saturation levels of fatty acids and ATP content in L. edodes mycelium under heat stress. This study proposed a regulatory network of 2,4-D in regulating L. edodes response to heat stress, providing a theoretical basis for improving L. edodes thermotolerance, and facilitating the understanding of the molecular mechanism of exogenous hormones in alleviating abiotic stress damage to macrofungi.