Synergistic Effects of Ad-Libitum Low-Dose Fructose Drinking and Low-Dose Streptozotocin Treatment in Wistar Rats: A Mild Model of Type 2 Diabetes

To develop a convenient animal model of T2D by pretreatment with low-dose 10% w/v fructose (FRC) solution followed by the injection of low doses of streptozotocin (STZ) in Wistar rats. For this 8-week experimental study; rats were first fed a standard chow ad-libitum diet and either tap water (n=40)...

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Published in:Acta medica Iranica 2017-05, Vol.55 (5), p.304-310
Main Authors: Sadeghi, Asie, Beigy, Maani, Alizadeh, Samira, Mazloom, Hossein, Vakili, Sanaz, Ahmadi, Saideh, Meshkani, Reza
Format: Article
Language:English
Subjects:
Animals
Blood Glucose
Diabetes
Diabetes Mellitus, Experimental - physiopathology
Diabetes Mellitus, Type 2 - physiopathology
Diet
Disease Models, Animal
Experimental animal model
Fructose
Fructose - administration & dosage
Hyperglycemia
Hyperglycemia - physiopathology
Insulin Resistance
Male
Rats
Rats, Wistar
Rodents
Streptozocin - administration & dosage
Streptozotocin
Type 2 diabetes
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container_title Acta medica Iranica
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creator Sadeghi, Asie
Beigy, Maani
Alizadeh, Samira
Mazloom, Hossein
Vakili, Sanaz
Ahmadi, Saideh
Meshkani, Reza
description To develop a convenient animal model of T2D by pretreatment with low-dose 10% w/v fructose (FRC) solution followed by the injection of low doses of streptozotocin (STZ) in Wistar rats. For this 8-week experimental study; rats were first fed a standard chow ad-libitum diet and either tap water (n=40) or 10% w/v FRC solution (n=40) for 4 weeks. Next, rats in each category were randomly allocated to 4 subgroups (n=10 each) of low-dose STZ (25,35, and 45 mg/kg). The final mean fasting blood sugar (FBG) of FRC+STZ45 (197±55.87 mg/dl) were significantly higher than that of the STZ45 (P=0.015) and FRC (P=0.019) groups. FRC+STZ45 showed the highest insulin resistance demonstrated by insulin tolerance test [area under the curve (AUC) of insulin tolerance test; P
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subjects Animals
Blood Glucose
Diabetes
Diabetes Mellitus, Experimental - physiopathology
Diabetes Mellitus, Type 2 - physiopathology
Diet
Disease Models, Animal
Experimental animal model
Fructose
Fructose - administration & dosage
Hyperglycemia
Hyperglycemia - physiopathology
Insulin Resistance
Male
Rats
Rats, Wistar
Rodents
Streptozocin - administration & dosage
Streptozotocin
Type 2 diabetes
title Synergistic Effects of Ad-Libitum Low-Dose Fructose Drinking and Low-Dose Streptozotocin Treatment in Wistar Rats: A Mild Model of Type 2 Diabetes
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