Label-free imaging and biochemical characterization of bovine sperm cells

A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH...

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Bibliographic Details
Published in:Biosensors (Basel) 2015-04, Vol.5 (2), p.141-157
Main Authors: Ferrara, Maria Antonietta, Di Caprio, Giuseppe, Managò, Stefano, De Angelis, Annalisa, Sirleto, Luigi, Coppola, Giuseppe, De Luca, Anna Chiara
Format: Article
Language:English
Subjects:
Animals
biophotonics
Cattle
digital holography microscopy
Holography - instrumentation
Holography - methods
Male
Raman imaging
Raman spectroscopy
Sex Preselection - methods
sex-identification
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
spermatozoa
Spermatozoa - abnormalities
Spermatozoa - cytology
X Chromosome
Y Chromosome
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Summary:A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios5020141