MiR-638 repressed vascular smooth muscle cell glycolysis by targeting LDHA

Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) accelerated vascular diseases progression, like atherosclerosis and restenosis. MicroRNAs were reported to participate in modulating diverse cellular processes. Here, we focused on exploring the role of miR-638 in VSMCs gly...

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Published in:Open medicine (Warsaw, Poland) Poland), 2019-01, Vol.14 (1), p.663-672
Main Authors: Chen, Shiyuan, Chen, Hu, Yu, Chaowen, Lu, Ran, Song, Tao, Wang, Xiaogao, Tang, Wenbo, Gao, Yong
Format: Article
Language:English
Subjects:
Atherosclerosis
Cancer
Cardiovascular disease
Cell growth
Glycolysis
Growth factors
Hospitals
Immunoglobulins
LDHA
Membranes
MicroRNAs
Migration and invasion
miR-638
PDGF-bb
Proliferation
Proteins
Smooth muscle
Vascular surgery
VSMCs
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Summary:Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) accelerated vascular diseases progression, like atherosclerosis and restenosis. MicroRNAs were reported to participate in modulating diverse cellular processes. Here, we focused on exploring the role of miR-638 in VSMCs glycolysis and underlying mechanism. Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability. Western blot assay was conducted to determine the expression of cell proliferation markers proliferating cell nuclear antigen (PCNA) and Ki-67, as well as Lactate dehydrogenase A (LDHA). VSMCs migration and invasion were evaluated by Transwell assay. Luciferase reporter gene assay and RNA immunoprecipitation were performed to validate the target relationship between miR-638 and LDHA. LDHA and miR-638 expression were also determined. Glycolysis of VSMCs was tested by corresponding Kits. Platelet-derived growth factor-bb (PDGF-bb) promoted the VSMCs viability and down-regulated miR-638. Overexpression of miR-638 inhibited cell proliferation, migration and invasion of VSMCs. LDHA was identified as a target of miR-638, and counter-regulated by miR-638. Loss of miR-638 attenuated the suppressor effects on the proliferation, migration and invasion of VSMCs induced by LDHA down-regulation. MiR-638 inhibited the glycolysis of VSMCs by targeting LDHA. MiR-638 is down-regulated by PDGF-bb treatment and suppressed the glycolysis of VSMCs via targeting LDHA.
ISSN:2391-5463
2391-5463
DOI:10.1515/med-2019-0077