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Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanosco...

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Published in:Nature communications 2021-02, Vol.12 (1), p.950-8, Article 950
Main Authors: Trofymchuk, Kateryna, Glembockyte, Viktorija, Grabenhorst, Lennart, Steiner, Florian, Vietz, Carolin, Close, Cindy, Pfeiffer, Martina, Richter, Lars, Schütte, Max L., Selbach, Florian, Yaadav, Renukka, Zähringer, Jonas, Wei, Qingshan, Ozcan, Aydogan, Lalkens, Birka, Acuna, Guillermo P., Tinnefeld, Philip
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cited_by cdi_FETCH-LOGICAL-c540t-6ea5be86446bc4090754a89f39f0ece30dc891a309916a5f7e5ea83e6256ff293
cites cdi_FETCH-LOGICAL-c540t-6ea5be86446bc4090754a89f39f0ece30dc891a309916a5f7e5ea83e6256ff293
container_end_page 8
container_issue 1
container_start_page 950
container_title Nature communications
container_volume 12
creator Trofymchuk, Kateryna
Glembockyte, Viktorija
Grabenhorst, Lennart
Steiner, Florian
Vietz, Carolin
Close, Cindy
Pfeiffer, Martina
Richter, Lars
Schütte, Max L.
Selbach, Florian
Yaadav, Renukka
Zähringer, Jonas
Wei, Qingshan
Ozcan, Aydogan
Lalkens, Birka
Acuna, Guillermo P.
Tinnefeld, Philip
description The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road. Single-molecule fluorescence currently requires specialized imaging equipment due to the low signal of a single emitter. Here the authors introduce NanoAntennas with Cleared HOtSpots (NACHOS) to boost the signal sufficient for detection of a single emitter by a smartphone, opening the door to point-of-care applications.
doi_str_mv 10.1038/s41467-021-21238-9
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However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road. Single-molecule fluorescence currently requires specialized imaging equipment due to the low signal of a single emitter. Here the authors introduce NanoAntennas with Cleared HOtSpots (NACHOS) to boost the signal sufficient for detection of a single emitter by a smartphone, opening the door to point-of-care applications.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33574261</pmid><doi>10.1038/s41467-021-21238-9</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9148-5837</orcidid><orcidid>https://orcid.org/0000-0003-0819-9248</orcidid><orcidid>https://orcid.org/0000-0001-9503-1819</orcidid><orcidid>https://orcid.org/0000-0002-5136-0669</orcidid><orcidid>https://orcid.org/0000-0003-4290-7770</orcidid><orcidid>https://orcid.org/0000-0002-8417-4168</orcidid><orcidid>https://orcid.org/0000-0003-1214-010X</orcidid><orcidid>https://orcid.org/0000-0003-3453-1320</orcidid><orcidid>https://orcid.org/0000-0002-2633-1639</orcidid><orcidid>https://orcid.org/0000-0001-8066-2677</orcidid><orcidid>https://orcid.org/0000-0003-2531-6506</orcidid><orcidid>https://orcid.org/0000-0003-3870-9069</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 2041-1723
ispartof Nature communications, 2021-02, Vol.12 (1), p.950-8, Article 950
issn 2041-1723
2041-1723
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_9db22d60e3b94597a65b769a9d0fd002
source PubMed Central(OA); Nature; Publicly Available Content (ProQuest); Springer Nature - nature.com Journals - Fully Open Access
subjects 631/61/350/59
639/624/399/354
639/925/926/1049
639/925/927/1021
639/925/930/2735
9/10
Antibiotic resistance
Antibiotics
Bioassays
Deoxyribonucleic acid
Diagnostic software
Diagnostic systems
DNA
DNA - chemistry
Drug Resistance, Bacterial
Emitters
Fluorescence
Humanities and Social Sciences
Humans
Klebsiella
Klebsiella pneumoniae - drug effects
Male
Microscopy
multidisciplinary
Nanoantennas
Nanostructures
Nanotechnology
Optical instruments
Point-of-Care Testing
Science
Science (multidisciplinary)
Serum - chemistry
Signal processing
Smartphone
Smartphones
title Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
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