Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells

The deleterious effects of aflatoxins, especially aflatoxin B1 (AFB1) which are widespread at all stages of food production, on the reproductive system have been widely reported in males. However, it is still far from fully understood about the toxic effect and molecular mechanism after exposure to...

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Published in:Ecotoxicology and environmental safety 2023-07, Vol.259, p.115063-115063, Article 115063
Main Authors: Han, Shuaiqi, Zhang, Hongyun, Liu, Xinyu, Wen, Fei, Li, Baiyu, Bie, Zhiwen, Qiu, Hehan, Hu, Jianhong
Format: Article
Language:English
Subjects:
AFB1
Aflatoxin B1 - toxicity
AMP-Activated Protein Kinases - metabolism
AMPK
Animals
Autophagy
Goats - metabolism
Lactate
Lactic Acid - metabolism
Male
Sertoli cells
Sertoli Cells - metabolism
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cited_by cdi_FETCH-LOGICAL-c474t-b8b33e5651ab10ec2347a544081eca0163897a0837026439e3bfbc36345bacaa3
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container_end_page 115063
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container_start_page 115063
container_title Ecotoxicology and environmental safety
container_volume 259
creator Han, Shuaiqi
Zhang, Hongyun
Liu, Xinyu
Wen, Fei
Li, Baiyu
Bie, Zhiwen
Qiu, Hehan
Hu, Jianhong
description The deleterious effects of aflatoxins, especially aflatoxin B1 (AFB1) which are widespread at all stages of food production, on the reproductive system have been widely reported in males. However, it is still far from fully understood about the toxic effect and molecular mechanism after exposure to AFB1 in various testicular cells, especially Sertoli cells (SCs) which provide various energy materials and support to the developing germ cells as nurse cells. In this work, we examined the effects of AFB1 in dairy goat SCs on lactate production and autophagy, and the role of autophagy on AFB1-induced reduction in lactate production. Mechanistically, AFB1 destroyed the energy balance and reduced the secretion of lactate in dairy goat SCs (P 
doi_str_mv 10.1016/j.ecoenv.2023.115063
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However, it is still far from fully understood about the toxic effect and molecular mechanism after exposure to AFB1 in various testicular cells, especially Sertoli cells (SCs) which provide various energy materials and support to the developing germ cells as nurse cells. In this work, we examined the effects of AFB1 in dairy goat SCs on lactate production and autophagy, and the role of autophagy on AFB1-induced reduction in lactate production. Mechanistically, AFB1 destroyed the energy balance and reduced the secretion of lactate in dairy goat SCs (P < 0.01), resulting in a reduced level of ATP (P < 0.01) and phosphorylation of AMPK (P < 0.01). Subsequently, activated AMPK triggers autophagy by directly phosphorylating ULK1 (P < 0.05). The enhancement of autophagy partially reversed the AFB1-induced decrease in lactate secretion by promoting glucose utilization (P < 0.01) and increasing the expression of proteins related to lactate secretion in dairy goat SCs (P < 0.05) such as GLUT1, GLUT3, LDHA, and MCT4. Collectively, our study suggests that AFB1 inhibits the secretion of lactate which supply for germ cell development by damaging the "Warburg-like" metabolism of dairy goat SCs. Moreover, autophagy contributes to the resistance of glucose metabolism damage induced by AFB1. All data generated or analyzed in this study are available from the corresponding authors upon request. [Display omitted] •AFB1 reduced lactate secretion in goat Sertoli cells.•AFB1 induced autophagy in Sertoli cells.•AFB1 activated the AMPK-ULK1 signal pathway.•Enhanced autophagy reversed the AFB1-induced reduction in lactate secretion.]]></description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2023.115063</identifier><identifier>PMID: 37229875</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>AFB1 ; Aflatoxin B1 - toxicity ; AMP-Activated Protein Kinases - metabolism ; AMPK ; Animals ; Autophagy ; Goats - metabolism ; Lactate ; Lactic Acid - metabolism ; Male ; Sertoli cells ; Sertoli Cells - metabolism</subject><ispartof>Ecotoxicology and environmental safety, 2023-07, Vol.259, p.115063-115063, Article 115063</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023 The Authors. Published by Elsevier Inc. 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[Display omitted] •AFB1 reduced lactate secretion in goat Sertoli cells.•AFB1 induced autophagy in Sertoli cells.•AFB1 activated the AMPK-ULK1 signal pathway.•Enhanced autophagy reversed the AFB1-induced reduction in lactate secretion.]]></description><subject>AFB1</subject><subject>Aflatoxin B1 - toxicity</subject><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>AMPK</subject><subject>Animals</subject><subject>Autophagy</subject><subject>Goats - metabolism</subject><subject>Lactate</subject><subject>Lactic Acid - metabolism</subject><subject>Male</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - metabolism</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kUtv1DAUhS0EotPCP0DISzaZ-pnHBgmq0laqxAJYWzf2zdRRJh5sZ9T59yRN6bIrS0fnnutzP0I-cbbljJeX_RZtwPG4FUzILeealfIN2XDWsEIort6SDeOqKkrN5Rk5T6lnjEmm9XtyJishmrrSG9Jfjw8wWnQUphwOD7A70YhHjGmRugFyePQj_c4LP7pp8Tm0ESEhneUBbIaMNC1a9mGkoaMOfDzRXYBMf2HMYfDU4jCkD-RdB0PCj8_vBfnz4_r31W1x__Pm7urbfWFVpXLR1q2UqOdvQ8sZWiFVBVopVnO0MDeXdVMBq2XFRKlkg7LtWitLqXQLFkBekLs11wXozSH6PcSTCeDNkxDizkDM3g5oGucqppzl0qEq0TZa19BUTY1MC2yWrC9r1iGGvxOmbPY-LW1gxDAlI2oxH5VLoWarWq02hpQidi-rOTMLMdOblZhZiJmV2Dz2-XnD1O7RvQz9RzQbvq4GnG929BhNsh4XZD6izXMp__qGf1D7qL0</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Han, Shuaiqi</creator><creator>Zhang, Hongyun</creator><creator>Liu, Xinyu</creator><creator>Wen, Fei</creator><creator>Li, Baiyu</creator><creator>Bie, Zhiwen</creator><creator>Qiu, Hehan</creator><creator>Hu, Jianhong</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20230701</creationdate><title>Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells</title><author>Han, Shuaiqi ; Zhang, Hongyun ; Liu, Xinyu ; Wen, Fei ; Li, Baiyu ; Bie, Zhiwen ; Qiu, Hehan ; Hu, Jianhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-b8b33e5651ab10ec2347a544081eca0163897a0837026439e3bfbc36345bacaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>AFB1</topic><topic>Aflatoxin B1 - toxicity</topic><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>AMPK</topic><topic>Animals</topic><topic>Autophagy</topic><topic>Goats - metabolism</topic><topic>Lactate</topic><topic>Lactic Acid - metabolism</topic><topic>Male</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Shuaiqi</creatorcontrib><creatorcontrib>Zhang, Hongyun</creatorcontrib><creatorcontrib>Liu, Xinyu</creatorcontrib><creatorcontrib>Wen, Fei</creatorcontrib><creatorcontrib>Li, Baiyu</creatorcontrib><creatorcontrib>Bie, Zhiwen</creatorcontrib><creatorcontrib>Qiu, Hehan</creatorcontrib><creatorcontrib>Hu, Jianhong</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Shuaiqi</au><au>Zhang, Hongyun</au><au>Liu, Xinyu</au><au>Wen, Fei</au><au>Li, Baiyu</au><au>Bie, Zhiwen</au><au>Qiu, Hehan</au><au>Hu, Jianhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>259</volume><spage>115063</spage><epage>115063</epage><pages>115063-115063</pages><artnum>115063</artnum><issn>0147-6513</issn><eissn>1090-2414</eissn><abstract><![CDATA[The deleterious effects of aflatoxins, especially aflatoxin B1 (AFB1) which are widespread at all stages of food production, on the reproductive system have been widely reported in males. However, it is still far from fully understood about the toxic effect and molecular mechanism after exposure to AFB1 in various testicular cells, especially Sertoli cells (SCs) which provide various energy materials and support to the developing germ cells as nurse cells. In this work, we examined the effects of AFB1 in dairy goat SCs on lactate production and autophagy, and the role of autophagy on AFB1-induced reduction in lactate production. Mechanistically, AFB1 destroyed the energy balance and reduced the secretion of lactate in dairy goat SCs (P < 0.01), resulting in a reduced level of ATP (P < 0.01) and phosphorylation of AMPK (P < 0.01). Subsequently, activated AMPK triggers autophagy by directly phosphorylating ULK1 (P < 0.05). The enhancement of autophagy partially reversed the AFB1-induced decrease in lactate secretion by promoting glucose utilization (P < 0.01) and increasing the expression of proteins related to lactate secretion in dairy goat SCs (P < 0.05) such as GLUT1, GLUT3, LDHA, and MCT4. Collectively, our study suggests that AFB1 inhibits the secretion of lactate which supply for germ cell development by damaging the "Warburg-like" metabolism of dairy goat SCs. Moreover, autophagy contributes to the resistance of glucose metabolism damage induced by AFB1. All data generated or analyzed in this study are available from the corresponding authors upon request. [Display omitted] •AFB1 reduced lactate secretion in goat Sertoli cells.•AFB1 induced autophagy in Sertoli cells.•AFB1 activated the AMPK-ULK1 signal pathway.•Enhanced autophagy reversed the AFB1-induced reduction in lactate secretion.]]></abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>37229875</pmid><doi>10.1016/j.ecoenv.2023.115063</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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subjects AFB1
Aflatoxin B1 - toxicity
AMP-Activated Protein Kinases - metabolism
AMPK
Animals
Autophagy
Goats - metabolism
Lactate
Lactic Acid - metabolism
Male
Sertoli cells
Sertoli Cells - metabolism
title Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells
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