Influence of ZnO Nanoparticles on the Properties of Ibuprofen-Loaded Alginate-Based Biocomposite Hydrogels with Potential Antimicrobial and Anti-Inflammatory Effects

• Published in: Pharmaceutics 2023-08, Vol.15 (9), p.2240
• Main Authors: Rata, Delia Mihaela, Cadinoiu, Anca Niculina, Daraba, Oana Maria, Gradinaru, Luiza Madalina, Atanase, Leonard Ionut, Ichim, Daniela Luminita
• Format: Article
• Language: English
• Subjects: alginate; Analysis; Antibiotics; Antimicrobial agents; Bacterial infections; Biocompatibility; Biological products; controlled drug release; Cytotoxicity; Drug resistance in microorganisms; Ethylenediaminetetraacetic acid; Extracellular matrix; Fibroblasts; Health aspects; Hyaluronic acid; Hydrogels; Ibuprofen; Injuries; Microscopy; Nanoparticles; Nonsteroidal anti-inflammatory drugs; Polymers; Sodium; Spectrum analysis; Viscosity; Wound healing; Zinc oxide; Zinc oxides; ZnO nanoparticles
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics15092240

Hydrogels are a favorable alternative to accelerate the burn wound healing process and skin regeneration owing to their capability of absorbing contaminated exudates. The bacterial infections that occur in burn wounds might be treated using different topically applied materials, but bacterial resistance to antibiotics has become a major problem worldwide. Therefore, the use of non-antibiotic treatments represents a major interest in current research. In this study, new antibiocomposite hydrogels with anti-inflammatory and antimicrobial properties based on hyaluronic acid (HA) and sodium alginate (AG) were obtained using 4-(4,6-dimethoxy-1,3,5-triazinyl-2)-4-methylmorpholinium chloride as an activator. The combination of Ibuprofen, a non-steroidal anti-inflammatory drug commonly used to reduce inflammation, fever and pain in the body, with zinc oxide nanoparticles (ZnO NPs) was used in this study aimed at creating a complex hydrogel with anti-inflammatory and antimicrobial action and capable of improving the healing process of wounds caused by burns. FTIR spectra confirmed the cross-linking of AG with HA as well as the successful incorporation of ZnO NPs. Using electronic microscopy, it was noticed that the morphology of hydrogels is influenced by the incorporation of ZnO nanoparticles. Moreover, the incorporation of ZnO nanoparticles into hydrogels also has an influence on the swelling behavior at both pH 7.4 and 5.4. In fact, the swelling rate is lower when the amounts of the activator, HA and ZnO NPs are high. A drug release rate of almost 100% was observed for hydrogels without ZnO NPs, whereas the addition of nanoparticles to hydrogels led to a decrease in the release rate to 68% during 24 h. Cellular viability tests demonstrated the non-cytotoxic behavior of the hydrogels without the ZnO NPs, whereas a weak to moderate cytotoxic effect was noticed for hydrogels with ZnO NPs. The hydrogels containing 4% and 5% ZnO NPs, respectively, showed good antimicrobial activity against the S. aureus strain. These preliminary data prove that these types of hydrogels can be of interest as biomaterials for the treatment of burn wounds.