Simultaneous measurements of chylomicron lipolysis and remnant removal using a doubly labeled artificial lipid emulsion: studies in normolipidemic and hyperlipidemic subjects

An artificial chylomicron-like lipid emulsion doubly labeled with tri[(N)3H]oleoylglycerol and cholesteryl [1-14C]oleate ([14C]CO) was infused intravenously into human subjects with the purpose of simultaneously measuring the plasma disappearance rates (residence time, RT) of [14C]CO, which represen...

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Bibliographic Details
Published in:Journal of lipid research 1994-01, Vol.35 (1), p.143-152
Main Authors: Nakandakare, E.R, Lottenberg, S.A, Oliveira, H.C.F, Bertolami, M.C, Vasconcelos, K.S, Sperotto, G, Quintao, E.C.R
Format: Article
Language:English
Subjects:
Adult
Aged
CARBONE
CARBONO
CHOLESTEROL
Cholesterol - blood
Cholesterol Esters - pharmacokinetics
CHYLOMICRON
Chylomicrons - metabolism
COLESTEROL
COLLOIDE
COLOIDES
CORPS GRAS
ESTER
ESTERES
Fat Emulsions, Intravenous - pharmacokinetics
Female
GENERO HUMANO
GENRE HUMAIN
GRASAS
Humans
Hypercholesterolemia - metabolism
Hyperlipidemias - metabolism
ISOTOPE RADIOACTIF
LIPOLISIS
LIPOLYSE
Lipolysis
Lipoproteins, VLDL - blood
Male
Middle Aged
QUILOMICRONES
RADIOISOTOPOS
TECHNIQUE DES TRACEURS
TECNICAS DE TRAZADORES
TRASTORNOS METABOLICOS
TRIGLICERIDOS
TRIGLYCERIDE
Triglycerides - blood
Triolein - pharmacokinetics
TRITIO
TRITIUM
TROUBLE DU METABOLISME
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Description
Summary:An artificial chylomicron-like lipid emulsion doubly labeled with tri[(N)3H]oleoylglycerol and cholesteryl [1-14C]oleate ([14C]CO) was infused intravenously into human subjects with the purpose of simultaneously measuring the plasma disappearance rates (residence time, RT) of [14C]CO, which represents solely the splanchnic organ uptake of the remnant chylomicron core, and of [3H]TO, which combines the remnant disappearance with the shedding off of chylomicron triglycerides by the action of lipoprotein lipase. Thus, the fraction of the particle triglyceride content that is removed before the remnant is taken up is expressed as a delipidation index (...). The present procedure has an advantage over the use of chylomicrons labeled with retinyl ester or radioactive triglycerides alone that represent, respectively, the chylomicron remnant or the whole particle metabolism only. When normal subjects as well as primary hyperlipidemic subjects were studied, the plasma triglyceride concentration was directly related to [14C]CO RT and [3H]TO RT, but inversely related to the delipidation index. There may be different patterns of relations between these parameters of chylomicron metabolism in primary and in secondary hyperlipidemias, as well as under the action of drugs that influence the metabolism of lipoproteins
ISSN:0022-2275
1539-7262
DOI:10.1016/S0022-2275(20)40120-8