Evaluating Thera-101 as a Low-Volume Resuscitation Fluid in a Model of Polytrauma

• Published in: International journal of molecular sciences 2022-10, Vol.23 (20), p.12664
• Main Authors: Shah, Jessica Stukel, Macaitis, Joseph, Lundquist, Bridney, Johnstone, Brian, Coleman, Michael, Jefferson, Michelle A., Glaser, Jacob, Rodriguez, Annette R., Cardin, Sylvain, Wang, Heuy-Ching, Burdette, Alexander
• Format: Article
• Language: English
• Subjects: Animals; Blood; cell-based therapy; Corpus callosum; Cytokines; Diffusion; Enzymes; Fornix; Growth factors; Heart rate; Hemorrhage; Hemorrhagic shock; Histology; Immunohistochemistry; Inflammation; Injury prevention; Kinases; Liver; Magnetic resonance imaging; Mesenchyme; Metabolism; Mortality; Neuroimaging; neuroprotection; organ damage; Plasma; Potential fields; Proteins; Respiration; Resuscitation; Secretome; Stromal cells; Trauma; Traumatic brain injury
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232012664

Traumatic brain injury (TBI) and hemorrhage remain challenging to treat in austere conditions. Developing a therapeutic to mitigate the associated pathophysiology is critical to meet this treatment gap, especially as these injuries and associated high mortality are possibly preventable. Here, Thera-101 (T-101) was evaluated as low-volume resuscitative fluid in a rat model of TBI and hemorrhage. The therapeutic, T-101, is uniquely situated as a TBI and hemorrhage intervention. It contains a cocktail of proteins and microvesicles from the secretome of adipose-derived mesenchymal stromal cells that can act on repair and regenerative mechanisms associated with poly-trauma. T-101 efficacy was determined at 4, 24, 48, and 72 h post-injury by evaluating blood chemistry, inflammatory chemo/cytokines, histology, and diffusion tensor imaging. Blood chemistry indicated that T-101 reduced the markers of liver damage to Sham levels while the levels remained elevated with the control (saline) resuscitative fluid. Histology supports the potential protective effects of T-101 on the kidneys. Diffusion tensor imaging showed that the injury caused the most damage to the corpus callosum and the fimbria. Immunohistochemistry suggests that T-101 may mitigate astrocyte activation at 72 h. Together, these data suggest that T-101 may serve as a potential field deployable low-volume resuscitation therapeutic.