Real-time PCR quantification of plasma DNA in non-small cell lung cancer patients and healthy controls

Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to dis...

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Bibliographic Details
Published in:European journal of medical research 2009-12, Vol.14 Suppl 4 (Suppl 4), p.237-240
Main Authors: Szpechcinski, A, Dancewicz, M, Kopinski, P, Kowalewski, J, Chorostowska-Wynimko, J
Format: Article
Language:English
Subjects:
Analysis
Cancer genetics
Carcinoma, Non-Small-Cell Lung - genetics
Care and treatment
DNA
DNA - blood
DNA quantification
DNA, Neoplasm - blood
free-circulating DNA
Genetic research
Humans
lung cancer
Lung Neoplasms - genetics
Non-small cell lung cancer
plasma
Polymerase Chain Reaction - methods
real-time PCR
ROC Curve
Small cell lung cancer
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Summary:Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to distinguish between healthy subjects and non-small cell lung cancer (NSCLC) patients. Plasma samples were collected prospectively from 16 healthy volunteers and 30 untreated NSCLC patients (I-IIIA). Subsequently, free-circulating DNA extraction and quantitative real-time PCR analysis were performed. The values of plasma DNA concentration ranged from 0.9 up to 7.0 ng/ml in healthy individuals and from 1.5 up to 50 ng/ml in NSCLC patients before treatment. Cancer group showed several-fold higher mean free-circulating DNA concentration than that present in healthy subjects (mean 12.00 vs. 2.65 ng/ml; P
ISSN:0949-2321
2047-783X
2047-783X
DOI:10.1186/2047-783X-14-S4-237