Supercritical fluid extraction and chromatographic analysis (HRGC-FID and HRGC-MS) of Lupinus spp. alkaloids

The alkaloid extracts from Lupinus spp., obtained by conventional methods (maceration/sonication - solid phase extraction; maceration/sonication - liquid-liquid extraction) and SFE (supercritical fluid extraction) using CO2 and modified CO2 (CO2/MeOH, CO2/EtOH, CO2/iPrOH and CO2/H2O) were analysed b...

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Bibliographic Details
Published in:Journal of the Brazilian Chemical Society 2000-01, Vol.11 (5), p.495-501
Main Authors: Nossack, Ana C., Vilegas, Janete H. Y., Baer, Dietrich von, Lanças, Fernando M.
Format: Article
Language:English
Subjects:
alkaloids
CHEMISTRY, MULTIDISCIPLINARY
high resolution gas chromatography (HRGC)
Lupinus spp
supercritical fluid extraction (SFE)
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Summary:The alkaloid extracts from Lupinus spp., obtained by conventional methods (maceration/sonication - solid phase extraction; maceration/sonication - liquid-liquid extraction) and SFE (supercritical fluid extraction) using CO2 and modified CO2 (CO2/MeOH, CO2/EtOH, CO2/iPrOH and CO2/H2O) were analysed by HRGC-FID (high resolution gas chromatography - flame ionization detector) and HRGC-MS (high resolution gas chromatography - mass spectrometry). The HRGC-FID quantitative analyses were performed with an internal standard method for quantification of lupanine, multiflorine and a spartein-like alkaloid. HRGC-MS allowed identification of the chemical constituents (alkaloids and other compounds) from these extracts.
ISSN:0103-5053
1678-4790
0103-5053
DOI:10.1590/S0103-50532000000500011