Sex differences in mouse infralimbic cortex projections to the nucleus accumbens shell

• Published in: Biology of sex differences 2023-12, Vol.14 (1), p.87-21, Article 87
• Main Authors: Johnson, Caroline S, Chapp, Andrew D, Lind, Erin B, Thomas, Mark J, Mermelstein, Paul G
• Format: Article
• Language: English
• Subjects: Animals; Behavior; Brain; Brain slice preparation; Cerebral Cortex; Cocaine; Disease susceptibility; Drug use; Estrogens; Excitability; Female; Females; Gender differences; Glutamate; Glutamatergic transmission; Humans; Infralimbic cortex; Laboratory animals; Male; Males; Mice; Motivation; Neurons; Neurons - physiology; Nucleus accumbens; Nucleus Accumbens - physiology; Nucleus accumbens shell; Reinforcement; Reward; Self-stimulation; Sex Characteristics; Sex differences; Spiny neurons; Substance abuse; Substance use; Substance use disorder
• ISSN: 2042-6410; 2042-6410
• DOI: 10.1186/s13293-023-00570-3

The nucleus accumbens (NAc) is an important region in motivation and reward. Glutamatergic inputs from the infralimbic cortex (ILC) to the shell region of the NAc (NAcSh) have been implicated in driving the motivation to seek reward through repeated action-based behavior. While this has primarily been studied in males, observed sex differences in motivational circuitry and behavior suggest that females may be more sensitive to rewarding stimuli. These differences have been implicated for the observed vulnerability in women to substance use disorders. We used an optogenetic self-stimulation task in addition to ex vivo electrophysiological recordings of NAcSh neurons in mouse brain slices to investigate potential sex differences in ILC-NAcSh circuitry in reward-seeking behavior. Glutamatergic neurons in the ILC were infected with an AAV delivering DNA encoding for channelrhodopsin. Entering the designated active corner of an open field arena resulted in photostimulation of the ILC terminals in the NAcSh. Self-stimulation occurred during two consecutive days of testing over three consecutive weeks: first for 10 Hz, then 20 Hz, then 30 Hz. Whole-cell recordings of medium spiny neurons in the NAcSh assessed both optogenetically evoked local field potentials and intrinsic excitability. Although both sexes learned to seek the active zone, within the first day, females entered the zone more than males, resulting in a greater amount of photostimulation. Increasing the frequency of optogenetic stimulation amplified female reward-seeking behavior. Males were less sensitive to ILC stimulation, with higher frequencies and repeated days required to increase male reward-seeking behavior. Unexpectedly, ex vivo optogenetic local field potentials in the NAcSh were greater in slices from male animals. In contrast, female medium-spiny neurons (MSNs) displayed significantly greater intrinsic neuronal excitability. Taken together, these data indicate that there are sex differences in the motivated behavior driven by glutamate within the ILC-NAcSh circuit. Though glutamatergic signaling was greater in males, heightened intrinsic excitability in females appears to drive this sex difference.