Preliminary studies on validation of calu-3 cell line as a model for screening respiratory mucosa irritation and toxicity

There is need to develop reproducible methods and experimental models for screening mucosal irritation and toxicity for drugs and pharmaceutical excipients. The aim of this study was to validate Calu-3 cell line as a model for screening respiratory irritation and toxicity of drugs and excipients. Ei...

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Bibliographic Details
Published in:Pharmaceutics 2014-06, Vol.6 (2), p.268-280
Main Authors: Ihekwereme, Chibueze, Esimone, Charles, Shao, Di, Agu, Remigius U
Format: Article
Language:English
Subjects:
Calu-3 cells
Drug therapy
MTT
mucosal toxicity
Mucous membrane
Toxicity
toxicity prediction
Validation studies
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Summary:There is need to develop reproducible methods and experimental models for screening mucosal irritation and toxicity for drugs and pharmaceutical excipients. The aim of this study was to validate Calu-3 cell line as a model for screening respiratory irritation and toxicity of drugs and excipients. Eighteen test compounds were selected according to their irritation potential and European Centre for the Validation of Alternative Methods (ECVAM) guidelines. Cell toxicity and irritation was determined using MTT assay. Data analysis and interpretation were done using modified ECVAM approach; where replicate values met acceptance criteria if percent relative standard deviation (RSD) of the raw data is
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics6020268