Nisin Z attenuates lipopolysaccharide-induced mastitis by inhibiting the ERK1/2 and p38 mitogen-activated protein kinase signaling pathways

Nisin Z is a possible alternative for treating bovine mastitis by inhibiting mastitis-causing pathogens and having anti-inflammatory activity. However, the anti-inflammatory mechanism of nisin Z on mastitis is unknown. Our study aimed to investigate the mechanisms of nisin Z on mastitis. Our results...

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Bibliographic Details
Published in:Journal of dairy science 2022-04, Vol.105 (4), p.3530-3543
Main Authors: Huang, Fuqing, Teng, Kunling, Liu, Yayong, Wang, Tianwei, Xia, Tianqi, Yun, Fangfei, Zhong, Jin
Format: Article
Language:English
Subjects:
Animals
blood-milk barrier
Cattle
Cattle Diseases
Female
Lipopolysaccharides - pharmacology
MAP Kinase Signaling System
mastitis
Mastitis - chemically induced
Mastitis - drug therapy
Mastitis - veterinary
Mice
mitogen-activated protein kinase
NF-kappa B - metabolism
Nisin - analogs & derivatives
nisin Z
p38 Mitogen-Activated Protein Kinases - metabolism
Signal Transduction
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Summary:Nisin Z is a possible alternative for treating bovine mastitis by inhibiting mastitis-causing pathogens and having anti-inflammatory activity. However, the anti-inflammatory mechanism of nisin Z on mastitis is unknown. Our study aimed to investigate the mechanisms of nisin Z on mastitis. Our results showed that nisin Z inhibited the activation of the ERK1/2 and p38 mitogen-activated protein kinase (MAPK) signaling pathway, decreased the release of pro-inflammatory cytokines (i.e., tumor necrosis factor-α, IL-1β, and IL-6), and increased the anti-inflammatory cytokine (IL-10) in lipopolysaccharide (LPS)-induced MCF10A cells. After intraperitoneal injection, nisin Z significantly decreased inflammatory cell infiltration in the mammary gland, as well as decreased myeloperoxidase and pro-inflammatory cytokines in serum and mammary gland. Western blot analysis revealed that nisin Z also dramatically suppressed the activation of the ERK1/2 and p38 MAPK signaling pathways in LPS-induced mastitis mice. We also found that nisin Z treatment could enhance the blood-milk barrier. In summary, our study demonstrated that nisin Z exerted an anti-inflammatory effect by inhibiting the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier on LPS-induced mastitis.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2021-21356