Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice

Aims. We have shown that growth hormone (GH) treatment poststroke increases neuroplasticity in peri-infarct areas and the hippocampus, improving motor and cognitive outcomes. We aimed to explore the mechanisms of GH treatment by investigating how GH modulates pathways known to induce neuroplasticity...

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Bibliographic Details
Published in:Neural plasticity 2022, Vol.2022, p.9983042-13
Main Authors: Sanchez-Bezanilla, Sonia, Beard, Daniel J., Hood, Rebecca J., Åberg, N. David, Crock, Patricia, Walker, Frederick R., Nilsson, Michael, Isgaard, Jörgen, Ong, Lin Kooi
Format: Article
Language:English
Subjects:
amyloid-beta accumulation
Animal cognition
Animals
Antibodies
Brain - metabolism
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - metabolism
gh treatment
Growth factors
Growth Hormone
Growth hormones
Human Growth Hormone - metabolism
in-vivo
Infarction - metabolism
insulin
mammalian target
Mammals
Mice
Neurodegeneration
Neuroplasticity
neuroprotection
Neurosciences
Neurosciences & Neurology
neurotrophic factor
Neurovetenskaper
Phosphorylation
Proteins
rat
Ribosomal Protein S6 Kinases, 70-kDa - metabolism
secondary neurodegeneration
Stroke - drug therapy
Stroke - metabolism
synaptic plasticity
TOR Serine-Threonine Kinases - metabolism
Traumatic brain injury
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Summary:Aims. We have shown that growth hormone (GH) treatment poststroke increases neuroplasticity in peri-infarct areas and the hippocampus, improving motor and cognitive outcomes. We aimed to explore the mechanisms of GH treatment by investigating how GH modulates pathways known to induce neuroplasticity, focusing on association between brain-derived neurotrophic factor (BDNF) and mammalian target of rapamycin (mTOR) in the peri-infarct area, hippocampus, and thalamus. Methods. Recombinant human growth hormone (r-hGH) or saline was delivered (0.25 μl/hr, 0.04 mg/day) to mice for 28 days, commencing 48 hours after photothrombotic stroke. Protein levels of pro-BDNF, total-mTOR, phosphorylated-mTOR, total-p70S6K, and phosporylated-p70S6K within the peri-infarct area, hippocampus, and thalamus were evaluated by western blotting at 30 days poststroke. Results. r-hGH treatment significantly increased pro-BDNF in peri-infarct area, hippocampus, and thalamus (p
ISSN:2090-5904
1687-5443
DOI:10.1155/2022/9983042