A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism

Primary methods play an important role in metrology. They can be used for the value assignment of certified reference materials, enabling the accuracy and comparability of the measurement. A novel potential primary method for enantiomer quantitation based on high-performance liquid chromatography-ci...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.7390-11, Article 7390
Main Authors: Luo, Yi, Wu, Liqing, Yang, Bin, Jin, Youxun, Zheng, Kangle, He, Zhangjing
Format: Article
Language:English
Subjects:
631/45
631/45/612/1221
82/16
Chromatography
Circular dichroism
Enantiomers
High-performance liquid chromatography
Humanities and Social Sciences
Insulin
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Mathematical models
multidisciplinary
Phenylalanine
Quantitation
Science
Science (multidisciplinary)
Scientific imaging
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Summary:Primary methods play an important role in metrology. They can be used for the value assignment of certified reference materials, enabling the accuracy and comparability of the measurement. A novel potential primary method for enantiomer quantitation based on high-performance liquid chromatography-circular dichroism is described using L-phenylalanine as an example. The optimal quantitation range of L-Phe was from 0.1 mg/g to 1.2 mg/g, where both the relative bias and method variance were lower than 1%. The LOD and LOQ were 4 μg/g and 30 μg/g, respectively. The proposed method was also applied to the determination of the mass fraction of pure porcine insulin in solid. The average mass fraction obtained was 0.922 g/g with a RSD of 1.5%, and the associated relative uncertainty is 3.8% ( k  = 2), which agreed well with that obtained from the traditional isotope dilution mass spectrometry method. The LOD and LOQ for insulin quantitation were found to be 0.12 mg/g and 0.44 mg/g, respectively. The proposed method can be entirely described and understood by equations and a complete uncertainty statement can be defined in SI units.Therefore, it may be a potential primary method useful for the quantification of chiral compounds and proteins, and a supplementary method to the traditional isotope dilution mass spectrometry approach.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-25682-4