Aerobactin-Mediated Iron Acquisition Enhances Biofilm Formation, Oxidative Stress Resistance, and Virulence of Yersinia pseudotuberculosis

Aerobactin is a citrate-hydroxamate siderophore that is critical for the virulence of pathogenic enteric bacteria. However, although the aerobactin-producing iucABCD - iutA operon is distributed widely in the genomes of Yersinia species, none of the pathogenic Yersinia spp. was found to produce aero...

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Bibliographic Details
Published in:Frontiers in microbiology 2021-07, Vol.12, p.699913-699913
Main Authors: Li, Changfu, Pan, Damin, Li, Mengyuan, Wang, Yao, Song, Luting, Yu, Danyang, Zuo, Yuxin, Wang, Kenan, Liu, Yuqi, Wei, Zhiyan, Lu, Zhiqiang, Zhu, Lingfang, Shen, Xihui
Format: Article
Language:English
Subjects:
aerobactin
Fur
iron acquisition
Microbiology
oxidative stress
siderophore
Yersinia pseudotuberculosis
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Summary:Aerobactin is a citrate-hydroxamate siderophore that is critical for the virulence of pathogenic enteric bacteria. However, although the aerobactin-producing iucABCD - iutA operon is distributed widely in the genomes of Yersinia species, none of the pathogenic Yersinia spp. was found to produce aerobactin. Here, we showed that the iucABCD - iutA operon in the food-borne enteric pathogen Yersinia pseudotuberculosis YPIII is a functional siderophore system involved in iron acquisition. The expression of the operon was found to be directly repressed by the ferric uptake regulator (Fur) in an iron concentration-dependent manner. In addition, we demonstrated that the aerobactin-mediated iron acquisition contributes to bacterial growth under iron-limited conditions. Moreover, we provided evidence that aerobactin plays important roles in biofilm formation, resistance to oxidative stress, ROS removal, and virulence of Y. pseudotuberculosis . Overall, our study not only uncovered a novel strategy of iron acquisition in Y. pseudotuberculosis but also highlighted the importance of aerobactin in the pathogenesis of Y. pseudotuberculosis .
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.699913