Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle

• Published in: E3S web of conferences 2023-01, Vol.462, p.1018
• Main Authors: Safina, Natalia, Shakirov, Shamil, Gaynutdinova, Elza, Mukhanina, Ekaterina, Shayakhmetova, Lilia, Bagavieva, Elmira, Fattakhova, Zilya, Akhmetov, Tahir, Zagidullin, Lenar, Haertdinov, Ravil
• Format: Article
• Language: English
• ISSN: 2267-1242; 2267-1242
• DOI: 10.1051/e3sconf/202346201018

The study examined the polymorphism of the glutathione peroxidase-1 gene ( GPX-1 , g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) and the Mukhametshin Z.Z. Peasant Farm Enterprise (PFE) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus GPX-1-Bsc4 I gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (C and T) and 3 genotypes (CC, TC and TT) were identified. The classification carried out by PIC values in two populations of Holstein cattle showed that there is a moderate polymorphism of the GPX-1 gene. A negative inbreeding coefficient (F) indicates excessive outbreeding in both populations. In both populations, the numerical advantage of heterozygous genotype TC cows is 60.1 and 56.7 % of the total livestock. Indigenous breeding cows, compared with imported cattle had higher: GPx activity, AST, ALT, cholesterol level, total protein and albumin fraction. Analysis of the association between the GPX-1 gene polymorphism and the GPx enzyme level indicates that the activity of this enzyme varies depending on the genotype, and there is also a variation in the biochemical parameters of the Holstein cattle blood serum.