Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology

Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by em...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2015-01, Vol.2015 (2015), p.1-8
Main Authors: Li, Wanyi, Tian, Hao, Li, Zhimin, Shi, Yao, Chu, Bowen, Wang, Yuanzhong
Format: Article
Language:English
Subjects:
Chemical compounds
Chemical properties
Chinese medicine
Chromatography
Disorders
Extraction
Extraction processes
Identification and classification
Mathematical analysis
Mathematical models
Medicine, Chinese
Optimization
Phytochemicals
Raw materials
Science
Solvents
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Summary:Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values ( 98.6 1 ± 0.6 1 ).
ISSN:2090-8865
2090-8873
DOI:10.1155/2015/819067