Single-base resolution quantitative genome methylation analysis in the model bacterium Helicobacter pylori by enzymatic methyl sequencing (EM-Seq) reveals influence of strain, growth phase, and methyl homeostasis

• Published in: BMC biology 2024-05, Vol.22 (1), p.125-125, Article 125
• Main Authors: Patel, Lubna, Ailloud, Florent, Suerbaum, Sebastian, Josenhans, Christine
• Format: Article
• Language: English
• Subjects: Analysis; Bacteria; Cytosine; Cytosine - metabolism; Deoxyribonucleic acid; DNA; DNA Methylation; DNA methyltransferases; DNA sequencing; Environmental conditions; Enzymes; Epigenetic changes; Epigenetic inheritance; Epigenetics; Gene sequencing; Genome, Bacterial - genetics; Genomes; Genomic analysis; Genomics; Growth; Helicobacter pylori; Helicobacter pylori - genetics; High-Throughput Nucleotide Sequencing - methods; High-throughput sequencing; Homeostasis; Methionine; Methods; Methylation; Methylome; Methyltransferases; Mutants; Next-generation sequencing; Phenotypic variations; Scientific equipment and supplies industry; Sequence Analysis, DNA - methods
• ISSN: 1741-7007; 1741-7007
• DOI: 10.1186/s12915-024-01921-1

Bacterial epigenetics is a rapidly expanding research field. DNA methylation by diverse bacterial methyltransferases (MTases) contributes to genomic integrity and replication, and many recent studies extended MTase function also to global transcript regulation and phenotypic variation. Helicobacter pylori is currently one of those bacterial species which possess the highest number and the most variably expressed set of DNA MTases. Next-generation sequencing technologies can directly detect DNA base methylation. However, they still have limitations in their quantitative and qualitative performance, in particular for cytosine methylation. As a complementing approach, we used enzymatic methyl sequencing (EM-Seq), a technology recently established that has not yet been fully evaluated for bacteria. Thereby, we assessed quantitatively, at single-base resolution, whole genome cytosine methylation for all methylated cytosine motifs in two different H. pylori strains and isogenic MTase mutants. EM-Seq reliably detected both C and C methylation. We demonstrated that three different active cytosine MTases in H. pylori provide considerably different levels of average genome-wide single-base methylation, in contrast to isogenic mutants which completely lost specific motif methylation. We found that strain identity and changed environmental conditions, such as growth phase and interference with methyl donor homeostasis, significantly influenced quantitative global and local genome-wide methylation in H. pylori at specific motifs. We also identified significantly hyper- or hypo-methylated cytosines, partially linked to overlapping MTase target motifs. Notably, we revealed differentially methylated cytosines in genome-wide coding regions under conditions of methionine depletion, which can be linked to transcript regulation. This study offers new knowledge on H. pylori global and local genome-wide methylation and establishes EM-Seq for quantitative single-site resolution analyses of bacterial cytosine methylation.