Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama

• Published in: Marine drugs 2020-09, Vol.18 (9), p.456
• Main Authors: Atencio, Librada A, Boya P, Cristopher A, Martin H, Christian, Mejía, Luis C, Dorrestein, Pieter C, Gutiérrez, Marcelino
• Format: Article
• Language: English
• Subjects: Animals; Anthozoa - microbiology; Anti-Infective Agents - metabolism; Anti-Infective Agents - pharmacology; Antiinfectives and antibacterials; antimicrobials; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - pharmacology; Biosynthesis; biosynthetic gene cluster; bromoalterochromides; Colour; Data Mining; Databases, Genetic; Depsipeptides - genetics; Depsipeptides - metabolism; Depsipeptides - pharmacology; Gene clusters; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Gene Regulatory Networks; Genes; genome mining; Genomes; Genomics; Metabolism; Metabolites; Microbiological strains; Microorganisms; MS/MS molecular networking; Multigene Family; National parks; Natural products; Networking; Panama; Parks, Recreational; Phylogeny; Pigmentation; Pseudoalteromonas; Pseudoalteromonas - genetics; Pseudoalteromonas - metabolism; Secondary Metabolism; Secondary metabolites; Strains (organisms); Submarine pipelines; Transcriptome
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md18090456

The marine bacterial genus is known for their ability to produce antimicrobial compounds. The metabolite-producing capacity of has been associated with strain pigmentation; however, the genomic basis of their antimicrobial capacity remains to be explained. In this study, we sequenced the whole genome of six strains (three pigmented and three non-pigmented), with the purpose of identifying biosynthetic gene clusters (BGCs) associated to compounds we detected via microbial interactions along through MS-based molecular networking. The genomes were assembled and annotated using the SPAdes and RAST pipelines and mined for the identification of gene clusters involved in secondary metabolism using the antiSMASH database. Nineteen BGCs were detected for each non-pigmented strain, while more than thirty BGCs were found for two of the pigmented strains. Among these, the groups of genes of nonribosomal peptide synthetases (NRPS) that code for bromoalterochromides stand out the most. Our results show that all strains possess BGCs for the production of secondary metabolites, and a considerable number of distinct polyketide synthases (PKS) and NRPS clusters are present in pigmented strains. Furthermore, the molecular networking analyses revealed two new molecules produced during microbial interactions: the dibromoalterochromides D/D' ( ).