Comparison on Protein Bioaccessibility of Soymilk Gels Induced by Glucono-δ-Lactone and Lactic Acid Bacteria

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-09, Vol.27 (19), p.6202
• Main Authors: Hui, Tianran, Tang, Ting, Gu, Xuan, Yuan, Zhen, Xing, Guangliang
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; Bacteria; bioaccessibility; Bioavailability; Digestibility; Digestion; Digestive system; Enzymes; Fermentation; Food; Gastrointestinal tract; Gels; Gels - chemistry; Gluconates; glucono-δ-lactone; Gluconolactone; in vitro digestion; Lactic acid; Lactic acid bacteria; Lactobacillales - metabolism; Lactobacillus; Lactones; Lactones - chemistry; Methods; Peptides; Physiological aspects; Plant-based beverages; Proteins; Soy Milk - chemistry; Soy products; soy protein; Soya bean milk; Soybean milk; Soybeans; Soymilk
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27196202

In this study, the protein bioaccessibility of soymilk gels produced by the addition of glu-cono-δ-lactone (GDL) and fermentation with lactic acid bacteria (LAB) was examined using an in vitro gastrointestinal simulated digestion model. The in vitro protein digestibility, soluble protein content, free amino acids contents, degree of hydrolysis, electrophoretic patterns, and peptide content were measured. The results suggested that acid-induced soymilk gel generated by GDL (SG) showed considerably reduced in vitro protein digestibility of 75.33 ± 1.00% compared to the soymilk gel induced by LAB (SL) of 80.57 ± 1.53% (p < 0.05). During the gastric digestion stage, dramatically higher (p < 0.05) soluble protein contents were observed in the SG (4.79−5.05 mg/mL) than that of SL (4.31−4.35 mg/mL). However, during the later intestinal digestion phase, the results were the opposite. At the end of the gastrointestinal digestion phase, the content of small peptides was not significantly different (p > 0.05) between the SL (2.15 ± 0.03 mg/mL) and SG (2.17 ± 0.01 mg/mL), but SL showed higher content of free amino acids (20.637 g/L) than that of SG (19.851 g/L). In general, soymilk gel induced by LAB had a higher protein bioaccessibility than the soymilk gel coagulated by GDL.