Green Radish Polysaccharide Prevents Alcoholic Liver Injury by Interfering with Intestinal Bacteria and Short-Chain Fatty Acids in Mice

• Published in: Foods 2024-11, Vol.13 (23), p.3733
• Main Authors: Geng, Xiong, Zhuang, Miaomiao, Tian, Weina, Shang, Huayan, Gong, Ziyi, Lv, Yanfang, Li, Jianrong
• Format: Article
• Language: English
• Subjects: Acetic acid; Alcohol use; Alcoholic beverages; alcoholic liver disease; Alcoholism; Alcohols; Antioxidants; Bacteria; Biochemical analysis; Butyric acid; Cholinergics; Chromatography; Damage prevention; Digestive system; Enzymes; Ethanol; Fatty acids; Gas chromatography; Gastrointestinal tract; Gluconeogenesis; Glycolysis; green radish; gut microbiota; Hepatitis; Inflammation; Injury analysis; Injury prevention; Interleukin 6; Intestinal microflora; Lipid metabolism; Lipids; Lipopolysaccharides; Liver; Liver cirrhosis; Liver diseases; Mass spectrometry; Mass spectroscopy; Metabolic pathways; Metabolism; Metabolomics; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Natural products; Next-generation sequencing; Oxidation; Oxidative stress; Permeability; polysaccharide; Polysaccharides; Prevention; Propionic acid; Proteins; Radishes; RNA; rRNA 16S; Saturated fatty acids; Sequences; short-chain fatty acid metabolism; Superoxide; Superoxide dismutase; Synapses; Tumor necrosis factor-TNF; Vibration
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13233733

This study aimed to ascertain the potential benefits of green radish polysaccharide (GRP) in treating alcoholic liver disease (ALD) in mice and explore its mechanism of action. Using biochemical analysis, high-throughput sequencing of gut microbiota, and gas chromatography-mass spectrometry to measure short-chain fatty acids (SCFAs) in feces, we found that GRP intervention significantly improved lipid metabolism and hepatic function in mice subjected to excessive alcohol intake. The GRP intervention reduced malondialdehyde levels by 66% and increased total superoxide dismutase levels by 22%, thereby mitigating alcohol-induced oxidative stress. Furthermore, GRP intervention in mice with alcohol consumption resulted in a reduction in tumor necrosis factor, interleukin 6, and lipopolysaccharide levels by 12%, 9%, and 25%, respectively, effectively attenuating alcoholic liver inflammation. 16S rRNA amplicon sequencing demonstrated that excessive alcohol consumption markedly altered the gut microbiota composition in mice. The GRP treatment resulted in a significant reduction in the number of beneficial bacteria ( and ) and an increase in the proportion of harmful bacteria ( and ). The metabolomic analyses of the SCFAs demonstrated an increase in the contents of SCFAs, acetic acid, propionic acid, and butyric acid, following GRP supplementation. Furthermore, the metabolic levels of cholinergic synapses and glycolysis/gluconeogenesis were found to be modulated. In conclusion, these findings suggest that GRP may attenuate alcohol-induced oxidative damage in the liver by modulating the gut microbiota and hepatic metabolic pathways. This may position GRP as a potential functional component for ALD prevention.