Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplificat...

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Bibliographic Details
Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2018-04, Vol.11 (2), p.35
Main Authors: Gu, Lide, Yan, Wanli, Liu, Le, Wang, Shujun, Zhang, Xu, Lyu, Mingsheng
Format: Article
Language:English
Subjects:
Binding sites
biosensor
Biosensors
cancer
Cancer therapies
clinical diagnostics
Deoxyribonucleic acid
DNA
DNA methylation
DNA polymerase
Enzymes
Growth factors
Kinases
Multilayered thin films
Phosphatase
Proteins
Review
rolling circle amplification (RCA)
Sensors
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Summary:Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic process that utilizes nuclease to generate long single stranded DNA (ssDNA) or RNA. The functional nucleic acid unit (aptamer, DNAzyme) could be replicated hundreds of times in a short period, and a lower LOD could be achieved if those units are combined with an enzymatic reaction, Surface Plasmon Resonance, electrochemical, or fluorescence detection, and other different kinds of biosensor. Multifarious RCA-based platforms have been developed to detect a variety of targets including DNA, RNA, SNP, proteins, pathogens, cytokines, micromolecules, and diseased cells. In this review, improvements in using the RCA technique for medical biosensors and biomedical applications were summarized and future trends in related research fields described.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph11020035