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Lung Inflammation Induced by Inactivated SARS-CoV-2 in C57BL/6 Female Mice Is Controlled by Intranasal Instillation of Vitamin D

The COVID-19 pandemic was triggered by the coronavirus SARS-CoV-2, whose peak occurred in the years 2020 and 2021. The main target of this virus is the lung, and the infection is associated with an accentuated inflammatory process involving mainly the innate arm of the immune system. Here, we descri...

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Published in:Cells (Basel, Switzerland) Switzerland), 2023-04, Vol.12 (7), p.1092
Main Authors: Fernandes de Souza, William Danilo, Zorzella-Pezavento, Sofia Fernanda Gonçalves, Ayupe, Marina Caçador, Salgado, Caio Loureiro, Oliveira, Bernardo de Castro, Moreira, Francielly, da Silva, Guilherme William, Muraro, Stefanie Primon, de Souza, Gabriela Fabiano, Proença-Módena, José Luiz, Araujo Junior, Joao Pessoa, Fonseca, Denise Morais da, Sartori, Alexandrina
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container_title Cells (Basel, Switzerland)
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creator Fernandes de Souza, William Danilo
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Ayupe, Marina Caçador
Salgado, Caio Loureiro
Oliveira, Bernardo de Castro
Moreira, Francielly
da Silva, Guilherme William
Muraro, Stefanie Primon
de Souza, Gabriela Fabiano
Proença-Módena, José Luiz
Araujo Junior, Joao Pessoa
Fonseca, Denise Morais da
Sartori, Alexandrina
description The COVID-19 pandemic was triggered by the coronavirus SARS-CoV-2, whose peak occurred in the years 2020 and 2021. The main target of this virus is the lung, and the infection is associated with an accentuated inflammatory process involving mainly the innate arm of the immune system. Here, we described the induction of a pulmonary inflammatory process triggered by the intranasal (IN) instillation of UV-inactivated SARS-CoV-2 in C57BL/6 female mice, and then the evaluation of the ability of vitamin D (VitD) to control this process. The assays used to estimate the severity of lung involvement included the total and differential number of cells in the bronchoalveolar lavage fluid (BALF), histopathological analysis, quantification of T cell subsets, and inflammatory mediators by RT-PCR, cytokine quantification in lung homogenates, and flow cytometric analysis of cells recovered from lung parenchyma. The IN instillation of inactivated SARS-CoV-2 triggered a pulmonary inflammatory process, consisting of various cell types and mediators, resembling the typical inflammation found in transgenic mice infected with SARS-CoV-2. This inflammatory process was significantly decreased by the IN delivery of VitD, but not by its IP administration, suggesting that this hormone could have a therapeutic potential in COVID-19 if locally applied. To our knowledge, the local delivery of VitD to downmodulate lung inflammation in COVID-19 is an original proposition.
doi_str_mv 10.3390/cells12071092
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The main target of this virus is the lung, and the infection is associated with an accentuated inflammatory process involving mainly the innate arm of the immune system. Here, we described the induction of a pulmonary inflammatory process triggered by the intranasal (IN) instillation of UV-inactivated SARS-CoV-2 in C57BL/6 female mice, and then the evaluation of the ability of vitamin D (VitD) to control this process. The assays used to estimate the severity of lung involvement included the total and differential number of cells in the bronchoalveolar lavage fluid (BALF), histopathological analysis, quantification of T cell subsets, and inflammatory mediators by RT-PCR, cytokine quantification in lung homogenates, and flow cytometric analysis of cells recovered from lung parenchyma. 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ispartof Cells (Basel, Switzerland), 2023-04, Vol.12 (7), p.1092
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2073-4409
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_9f7885ee5239495cb29e52976c7d0e7e
source Publicly Available Content Database; PubMed Central; Coronavirus Research Database
subjects Alfacalcidol
Animals
Asymptomatic
Bacterial pneumonia
Bronchus
Calcifediol
Care and treatment
Coronaviruses
COVID-19
Cytokine storm
Diagnosis
Dosage and administration
Drug delivery systems
Drug dosages
Drugs
Female
Flow cytometry
Humans
Immune system
Infections
Inflammation
Intranasal medication
Investigations
Lavage
lung
Lungs
Lymphocytes T
Methods
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nervous system
Neutrophils
Pandemics
Parenchyma
Pathogens
Pneumonia
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Transgenic mice
Vehicles
Viral infections
Vitamin D
Vitamin D - pharmacology
Vitamins
title Lung Inflammation Induced by Inactivated SARS-CoV-2 in C57BL/6 Female Mice Is Controlled by Intranasal Instillation of Vitamin D
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