Development of Salmon Sperm DNA/Regenerated Silk Bio-Based Films for Biomedical Studies on Human Keratinocyte HaCaT Cells under Solar Spectrum

In this study, we fabricated adhesive patches from silkworm-regenerated silk and DNA to safeguard human skin from the sun's rays. The patches are realized by exploiting the dissolution of silk fibers (e.g., silk fibroin (SF)) and salmon sperm DNA in formic acid and CaCl solutions. Infrared spec...

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Bibliographic Details
Published in:Journal of functional biomaterials 2023-05, Vol.14 (5), p.280
Main Authors: Ceccarini, Maria Rachele, Ripanti, Francesca, Raggi, Veronica, Paciaroni, Alessandro, Petrillo, Caterina, Comez, Lucia, Donato, Kevin, Bertelli, Matteo, Beccari, Tommaso, Valentini, Luca
Format: Article
Language:English
Subjects:
Absorption
Adhesives
Analysis
Biodegradable materials
Biological effects
biomedical devices
Biopolymers
Calcium chloride
cell metabolism
Cell viability
Circular dichroism
Deoxyribonucleic acid
Dichroism
DNA
DNA damage
DNA structure
Fibers
Formic acid
Genetic testing
Infrared spectroscopy
Keratinocytes
Medical research
Medicine, Experimental
Nanoparticles
Oxidative stress
Patches (structures)
Photovoltaic cells
regenerated silk
Salmon
Severe acute respiratory syndrome coronavirus 2
Shear strength
Shear tests
Silk
Silk fibroin
Silkworms
Skin cancer
Sperm
Stability analysis
Sustainable materials
Thermal analysis
Ultraviolet radiation
Vitamin D
Water absorption
Wound healing
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Summary:In this study, we fabricated adhesive patches from silkworm-regenerated silk and DNA to safeguard human skin from the sun's rays. The patches are realized by exploiting the dissolution of silk fibers (e.g., silk fibroin (SF)) and salmon sperm DNA in formic acid and CaCl solutions. Infrared spectroscopy is used to investigate the conformational transition of SF when combined with DNA; the results indicated that the addition of DNA provides an increase in the SF crystallinity. UV-Visible absorption and circular dichroism spectroscopy showed strong absorption in the UV region and the presence of B-form of DNA once dispersed in the SF matrix, respectively. Water absorption measurements as well as thermal dependence of water sorption and thermal analysis, suggested the stability of the fabricated patches. Biological results on cellular viability (MTT assay) of keratinocyte HaCaT cells after exposures to the solar spectrum showed that both SF and SF/DNA patches are photo-protective by increasing the cellular viability of keratinocytes after UV component exposure. Overall, these SF/DNA patches promise applications in wound dressing for practical biomedical purposes.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb14050280