Transcriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice

We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of the core oscillator we compared preBötC neurons derived from Dbx1-expressing progenitors that...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-08, Vol.7 (1), p.8669-8669, Article 8669
Main Authors: Hayes, John A., Kottick, Andrew, Picardo, Maria Cristina D., Halleran, Andrew D., Smith, Ronald D., Smith, Gregory D., Saha, Margaret S., Del Negro, Christopher A.
Format: Article
Language:English
Subjects:
631/378/2583
631/378/87
Brain stem
Chemoreception
Dbx1 protein
Developmental stages
Gene expression
Humanities and Social Sciences
Hypoxia
Interneurons
multidisciplinary
Narcotics
Neonates
Neural networks
Opioid receptors
Pax8 protein
Respiration
Science
Science (multidisciplinary)
Therapeutic applications
Transcription factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of the core oscillator we compared preBötC neurons derived from Dbx1-expressing progenitors that are respiratory rhythmogenic to neighbouring non-Dbx1-derived neurons, which support other respiratory and non-respiratory functions. Results in three categories are particularly salient. First, Dbx1 preBötC neurons express κ-opioid receptors in addition to μ-opioid receptors that heretofore have been associated with opiate respiratory depression, which may have clinical applications. Second, Dbx1 preBötC neurons express the hypoxia-inducible transcription factor Hif1a at levels three-times higher than non-Dbx1 neurons, which links core rhythmogenic microcircuits to O 2 -related chemosensation for the first time. Third, we detected a suite of transcription factors including Hoxa4 whose expression pattern may define the rostral preBötC border, Pbx3 that may influence ipsilateral connectivity, and Pax8 that may pertain to a ventrally-derived subset of Dbx1 preBötC neurons. These data establish the transcriptomic signature of the core respiratory oscillator at a perinatal stage of development.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-09418-4