Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State

Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) are the pluripotent stem cells (PSCs), derived from the inner cell mass (ICM) of preimplantation embryos at embryonic day 3.5 (E3.5) and postimplantation embryos at E5.5-E7.5, respectively. Depending on their environment, PSC...

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Bibliographic Details
Published in:Stem cells international 2021, Vol.2021, p.8818356-12
Main Authors: Devika, A. S., Montebaur, Anna, Saravanan, S., Bhushan, Raghu, Koch, Frederic, Sudheer, Smita
Format: Article
Language:English
Subjects:
Artificial chromosomes
Cell culture
Data analysis
Embryo cells
Embryonic stem cells
Embryos
Exposure
Genes
Genomes
Phosphatase
Pluripotency
Primitive streak
Stem cell transplantation
Stem cells
Transcription factors
Wnt protein
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Summary:Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) are the pluripotent stem cells (PSCs), derived from the inner cell mass (ICM) of preimplantation embryos at embryonic day 3.5 (E3.5) and postimplantation embryos at E5.5-E7.5, respectively. Depending on their environment, PSCs can exist in the so-called naïve (ESCs) or primed (EpiSCs) states. Exposure to EpiSC or human ESC (hESC) culture condition can convert mESCs towards an EpiSC-like state. Here, we show that the undifferentiated epiblast state is however not stabilized in a sustained manner when exposing mESCs to hESC or EpiSC culture condition. Rather, prolonged exposure to EpiSC condition promotes a transition to a primitive streak- (PS-) like state via an unbiased epiblast-like intermediate. We show that the Brachyury-positive PS-like state is likely promoted by endogenous WNT signaling, highlighting a possible species difference between mouse epiblast-like stem cells and human Embryonic Stem Cells.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2021/8818356