Crosstalk between the Arabidopsis Glutathione Peroxidase-Like 5 Isoenzyme (AtGPXL5) and Ethylene

Glutathione peroxidases (GPXs) are important antioxidant enzymes in animals. Plants contain GPX-like (GPXL) enzymes, which-in contrast to GPXs-contain cysteine in their active site instead of selenocysteine. Although several studies proved their importance in development and stress responses, their...

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Published in:International journal of molecular sciences 2022-05, Vol.23 (10), p.5749
Main Authors: Riyazuddin, Riyazuddin, Bela, Krisztina, Poór, Péter, Szepesi, Ágnes, Horváth, Edit, Rigó, Gábor, Szabados, László, Fehér, Attila, Csiszár, Jolán
Format: Article
Language:English
Subjects:
Abiotic stress
antioxidant mechanism
Antioxidants
Arabidopsis thaliana
Ascorbic acid
Biosynthesis
Carboxylic acids
Crosstalk
Enzymes
Ethylene
Evolution
Gene expression
Genotype & phenotype
Genotypes
Glutathione peroxidase
glutathione peroxidase-like 5 enzyme
Hydrogen peroxide
Hypocotyls
Mutants
Oxidative stress
Peroxidase
Phenotypes
Physiology
Plant growth
Polyamines
reactive oxygen species
Seedlings
Selenocysteine
Signal transduction
Thioredoxin
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Summary:Glutathione peroxidases (GPXs) are important antioxidant enzymes in animals. Plants contain GPX-like (GPXL) enzymes, which-in contrast to GPXs-contain cysteine in their active site instead of selenocysteine. Although several studies proved their importance in development and stress responses, their interaction with ethylene (ET) signalling is not known. Our aim was to investigate the involvement of AtGPXL5 in ET biosynthesis and/or signalling using mutant and cDNA-overexpressing (OX-AtGPXL5) lines. Four-day-old dark-grown seedlings had shorter hypocotyls and primary roots, while OX-AtGPXL5 seedlings exhibited a similar phenotype as wild type under normal conditions. Six-week-old OX-AtGPXL5 plants contained less H O and malondialdehyde, but higher polyamine and similar ascorbate- and glutathione contents and redox potential ( ) than the Col-0. One-day treatment with the ET-precursor 1-aminocyclopropane-1-carboxylic acid (ACC) induced the activity of glutathione- and thioredoxin peroxidases and some other ROS-processing enzymes. In the mutants, the became more oxidised; parallelly, it produced more ethylene after the ACC treatment than other genotypes. Although the enhanced ET evolution measured in the mutant can be the result of the increased ROS level, the altered expression pattern of ET-related genes both in the and OX-AtGPXL5 plants suggests the interplay between AtGPXL5 and ethylene signalling.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23105749