Seeding density is a crucial determinant for the in vivo vascularisation capacity of adipose tissue-derived microvascular fragments

Adipose tissue-derived microvascular fragments (ad-MVF) represent effective vascularisation units for the seeding of dermal substitutes. However, particularly in case of extensive skin defects, the required amounts of donor fat tissue for the harvesting of ad-MVF may not always be available. Therefo...

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Bibliographic Details
Published in:European cells & materials 2017-08, Vol.34, p.55-69
Main Authors: Später, T, Körbel, C, Frueh, F S, Nickels, R M, Menger, M D, Laschke, M W
Format: Article
Language:English
Subjects:
Adipose Tissue - blood supply
Adipose Tissue - metabolism
Animals
Antigens, CD - metabolism
dermal substitute
dorsal skinfold chamber
Epididymis - metabolism
Epithelium - metabolism
Erythrocytes - metabolism
Male
Mice, Inbred C57BL
Microscopy, Fluorescence
Microvessels - growth & development
Neovascularization, Physiologic
ntegra
photoacoustic imaging
Photoacoustic Techniques
Prostheses and Implants
skin
tissue engineering
Ultrasonics
vascularisation
wound healing
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Summary:Adipose tissue-derived microvascular fragments (ad-MVF) represent effective vascularisation units for the seeding of dermal substitutes. However, particularly in case of extensive skin defects, the required amounts of donor fat tissue for the harvesting of ad-MVF may not always be available. Therefore, we herein determined the lowest ad-MVF density needed to induce a sufficient vascularisation and incorporation of seeded implants. Collagen-glycosaminoglycan matrices (Integra®; diameter: 4 mm) were seeded with 15,000 (HD), 10,000 (MD) and 5,000 (LD) ad-MVF and implanted into full-thickness skin defects within mouse dorsal skinfold chambers, to analyse their in vivo vascularisation and incorporation. Intravital fluorescence microscopy showed a comparable vascularisation of HD and MD ad-MVF-seeded Integra®, which was significantly higher when compared to LD ad-MVF-seeded Integra®. As assessed by photoacoustic imaging, this was associated with an increased oxygenation of the implants. Additional histological and immunohistochemical analyses revealed an enhanced cellular infiltration, collagen content, microvessel density and epithelialisation of HD and MD ad-MVF-seeded Integra®, indicating a better incorporation compared to LD ad-MVF-seeded implants. These findings demonstrate that 80,000 ad-MVF/cm² is the least required density to guarantee an effective vascularisation of the dermal substitute.
ISSN:1473-2262
1473-2262
DOI:10.22203/eCM.v034a04