Effects of Alginate Concentration and Ovarian Cells on In Vitro Development of Mouse Preantral Follicles: A Factorial Study

In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. In the first step of this experimental study, preantral follicles were isolated from the ovaries of 2-week-old mice, encapsulated in t...

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Bibliographic Details
Published in:International journal of fertility & sterility 2020-01, Vol.13 (4), p.330-338
Main Authors: Jamalzaei, Parisa, Valojerdi, Mojtaba Rezazadeh, Montazeri, Leila, Baharvand, Hossein
Format: Article
Language:English
Subjects:
alginate
Follicles
hydrogel
Hydrogels
Insulin
Investigations
Original
ovarian cells
Ovaries
Penicillin
preantral follicle
Tissue engineering
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Summary:In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. In the first step of this experimental study, preantral follicles were isolated from the ovaries of 2-week-old mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% ALG hydrogels, and cultured for 14 days. The morphology, diameter, survival and antrum formation rates of the follicles and the maturation of the oocytes were evaluated during culture. In the second step, preantral follicles were cultured in the best chosen ALG concentration, in both the absence and presence of OCs. Following these steps, the amount of DNA fragmentation, the expression levels of connexin 37 and connexin 43 proteins, the secretion levels of estradiol, progesterone and androstenedione by the follicles and the quality of mature (MII) oocytes were assessed. Our data revealed that in the absence of OCs, follicles of 0.5% group showed a higher survival rate than the 0.75 and 1% groups (71.87 vs. 52.52 and 40%, respectively, P
ISSN:2008-076X
2008-0778
DOI:10.22074/ijfs.2020.5746