Structural characterization and hepatoprotective activity of an acidic polysaccharide from Ganoderma lucidum

• Published in: Food Chemistry: X 2022-03, Vol.13, p.100204-100204, Article 100204
• Main Authors: Chen, Shaodan, Guan, Xiaoying, Yong, Tianqiao, Gao, Xiong, Xiao, Chun, Xie, Yizhen, Chen, Diling, Hu, Huiping, Wu, Qingping
• Format: Article
• Language: English
• Subjects: (s) from the Special Issue on 5th International Symposium on Phytochemicals in Medicine and Food by Shao-Ping Nie and Jiaoyan Ren Ren; anion exchange chromatography; Anion-exchange chromatography (AEC); food chemistry; Ganoderma lucidum; Ganoderma lucidum polysaccharides; gels; Hepatoprotective; hepatoprotective effect; ionic strength; liver; molecular weight; permeability; pharmaceutical industry; Restraint stress; β-glucans
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2022.100204

•Ganoderma lucidum crude polysaccharide (GLP) exhibited protective effect on liver damage in mice caused by restraint stress through improving oxidative status.•Two polysaccharides, including a neutral β-glucan and an acidic β-glucan containing glucuronic acid were purified from GLP by anion-exchange chromatography (AEC) and gel filtration.•Acidic polysaccharide demonstrated stronger hepatoprotective effect in vitro compared to neutral polysaccharide.•Anion-exchange chromatography (AEC) is an effective technique for separate β-glucan into neutral and ionic fractions by different ionic strength buffer. In this study, Ganoderma lucidum crude polysaccharide (GLP) was found to have protective effect on liver damage in mice caused by restraint stress through improving oxidative status. Two polysaccharides, including a neutral β-glucan (GLPB2) and an acidic β-glucan (GLPC2) were purified from GLP through anion-exchange chromatography (AEC) combined with gel permeation. GLPC2, with an average molecular weight of 20.56 kDa, exhibited stronger hepatoprotective effect against H2O2-induced liver injury in HepG2 cells compared to GLPB2. Glycosidic residues and NMR analysis comprehensively revealed that GLPC2 contained d-Glcp-(1→, →3)-d-Glcp-(1→, →4)-d-Glcp-(1→, →6)-d-Glcp-(1→, →3, 6)-d-Glcp-(1 → and → 4)-d-GlcpA-(1 → . AEC can be an effective technique for separating β-glucans into neutral and acidic fractions by different ionic strength buffer. The findings provided a theoretical basis for the potential application of G. lucidum polysaccharides as a hepatoprotective in food and pharmaceutical industry.