Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective

Bacterial chromosomes have a single, unique replication origin (named ), from which DNA synthesis starts. This study describes methods of visualizing regions and the chromosome replication in single living bacterial cells in real-time. This review also discusses the impact of live cell imaging techn...

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Bibliographic Details
Published in:Frontiers in microbiology 2018-11, Vol.9, p.2819-2819
Main Authors: Trojanowski, Damian, Hołówka, Joanna, Zakrzewska-Czerwińska, Jolanta
Format: Article
Language:English
Subjects:
bacterial chromosome
Microbiology
oriC
replication initiation
replisome
single-cell
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Summary:Bacterial chromosomes have a single, unique replication origin (named ), from which DNA synthesis starts. This study describes methods of visualizing regions and the chromosome replication in single living bacterial cells in real-time. This review also discusses the impact of live cell imaging techniques on understanding of chromosome replication dynamics, particularly at the initiation step, in different species of bacteria.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.02819