Multiple-locus variable number of tandem repeat analysis (MLVA) of Irish verocytotoxigenic Escherichia coli O157 from feedlot cattle: uncovering strain dissemination routes

The identification of the routes of dissemination of Escherichia coli (E. coli) O157 through a cohort of cattle is a critical step to control this pathogen at farm level. The aim of this study was to identify potential routes of dissemination of E. coli O157 using Multiple-Locus Variable number of t...

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Bibliographic Details
Published in:BMC veterinary research 2008-01, Vol.4 (1), p.2-2
Main Authors: Murphy, Mary, Minihan, Donal, Buckley, James F, O'Mahony, Micheál, Whyte, Paul, Fanning, Séamus
Format: Article
Language:English
Subjects:
Animals
Cattle
Cattle Diseases - microbiology
Cattle Diseases - transmission
DNA
DNA Primers - chemistry
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
Escherichia coli
Escherichia coli Infections - microbiology
Escherichia coli Infections - transmission
Escherichia coli Infections - veterinary
Escherichia coli O157 - classification
Escherichia coli O157 - genetics
Escherichia coli O157 - isolation & purification
Feces - microbiology
Genetic aspects
Genetic Variation
Health aspects
Housing, Animal
Ireland
Phylogeny
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Ruminants
Soil Microbiology
Tandem Repeat Sequences - genetics
Water Microbiology
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Summary:The identification of the routes of dissemination of Escherichia coli (E. coli) O157 through a cohort of cattle is a critical step to control this pathogen at farm level. The aim of this study was to identify potential routes of dissemination of E. coli O157 using Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Thirty-eight environmental and sixteen cattle faecal isolates, which were detected in four adjacent pens over a four-month period were sub-typed. MLVA could separate these isolates into broadly defined clusters consisting of twelve MLVA types. Strain diversity was observed within pens, individual cattle and the environment. Application of MLVA is a broadly useful and convenient tool when applied to uncover the dissemination of E. coli O157 in the environment and in supporting improved on-farm management of this important pathogen. These data identified diverse strain types based on amplification of VNTR markers in each case.
ISSN:1746-6148
1746-6148
DOI:10.1186/1746-6148-4-2