Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection

G-quadruplexes have received significant attention in aptasensing due to their structural polymorphisms and unique binding properties. In this work, we exploited the fluorescence-quenching properties of G-quadruplex to develop a simple, fast, and sensitive platform for fluorescence detection of ochr...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-06, Vol.12 (6), p.423
Main Authors: Yang, Cheng, Chu, Xiaolin, Zeng, Li, Rhouati, Amina, Abbas, Fathimath, Cui, Shengnan, Lin, Daiqin
Format: Article
Language:English
Subjects:
Biosensors
Chloride
Design and construction
DNA sequencing
Electron transfer
Experiments
Fluorescein
Fluorescence
fluorescence aptasensor
G-quadruplex
Ions
label-free
Ligands
Measurement
Methods
Mycotoxins
Nucleic acid aptamers
Nucleotide sequencing
Ochratoxin A
photo-induced electron transfer
Potassium
Quenching
Selectivity
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Summary:G-quadruplexes have received significant attention in aptasensing due to their structural polymorphisms and unique binding properties. In this work, we exploited the fluorescence-quenching properties of G-quadruplex to develop a simple, fast, and sensitive platform for fluorescence detection of ochratoxin A (OTA) and potassium ions (K+) with a label-free fluorophore and quencher strategy. The quenching ability of G-quadruplex was confirmed during the recognition process after the formation of the G-quadruplex structure and the quenching of the labeled fluorescein fluorophore (FAM). The fluorescence-quenching mechanism was studied by introducing specific ligands of G-quadruplex to enhance the quenching effect, to show that this phenomenon is due to photo-induced electron transfer. The proposed fluorescence sensor based on G-quadruplex quenching showed excellent selectivity with a low detection limit of 0.19 nM and 0.24 µM for OTA and K+, respectively. Moreover, we demonstrated that our detection method enables accurate concentration determination of real samples with the prospect of practical application. Therefore, G-quadruplexes can be excellent candidates as quenchers, and the strategy implemented in the study can be extended to an aptasensor with G-quadruplex.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12060423