Cathodic adsorptive stripping voltammetric determination of Ribavirin in pharmaceutical dosage form, urine and serum

• Published in: Arabian journal of chemistry 2017-05, Vol.10 (S2), p.S2175-S2181
• Main Authors: Abdel Gaber, Ahmed A., Ahmed, Salwa A., Abdel Rahim, Asmaa M.
• Format: Article
• Language: English
• Subjects: Pharmaceutical formulation; Ribavirin; Square-wave adsorptive stripping voltammetry; Validation
• ISSN: 1878-5352; 1878-5379
• DOI: 10.1016/j.arabjc.2013.07.051

A sensitive, simple and rapid square-wave adsorptive stripping voltammetric method was developed and validated for the determination of Ribavirin in pharmaceutical formulations. The proposed method was based on the electrochemical reduction of Ribavirin at a hanging mercury drop electrode in Britton Robinson buffer at pH 10. A well-defined peak was observed at 880mV with 30s of accumulation time and 50mV of accumulation potential. Under these optimized conditions, the square-wave adsorptive stripping voltammetric peak current showed a linear correlation on drug concentration over the range of 1×10−10–2×10−7molL−1 with a correlation coefficient of 0.9995 for the proposed method. The detection and quantitation limits for this method were 2.02×10−10 and 6.80×10−10molL−1, respectively. The results obtained for intra-day and inter-day precision (as RSD %) were between 0.447% and 1.024%. This method was applied successfully for the determination of Ribavirin in its pharmaceutical dosage forms with mean recoveries of 99.68±0.13 with RSD % of 0.81% and 99.20±0.24 with RSD % of 0.49% for two concentrations 5×10−9 and 5×10−8molL−1, respectively for 200mg capsules. The results obtained from the developed square-wave adsorptive stripping voltammetric method were compared with those obtained by the analytical method reported in the literature.