Hippophae rhamnoides berry related Pichia kudriavzevii yeast volatiles modify behaviour of Rhagoletis batava flies

• Published in: Journal of advanced research 2020-01, Vol.21, p.71-77
• Main Authors: Mozūraitis, Raimondas, Aleknavičius, Dominykas, Vepštaitė-Monstavičė, Iglė, Stanevičienė, Ramunė, Emami, Seyedeh Noushin, Apšegaitė, Violeta, Radžiutė, Sandra, Blažytė-Čereškienė, Laima, Servienė, Elena, Būda, Vincas
• Format: Article
• Language: English
• Subjects: Gas chromatography-electroantennographic detection; Gas chromatography-mass spectrometry; Original; Pichia kudriavzevii; Rhagoletis batava; Solid phase micro extraction; Volatiles
• ISSN: 2090-1232; 2090-1224; 2090-1224
• DOI: 10.1016/j.jare.2019.08.001

[Display omitted] •Pichia kudriavzevii yeasts were isolated from ripe Hippophae rhamnoides berries.•Thirty-five yeast volatiles were identified from the headspace of P. kudriavzevii.•Esters and alcohols contributed by 32% and 66% to the total blend amount.•Ten of those volatiles elicited antenna responses of Rhagoletis batava flies.•Mixture of synthetic olfactory active compounds attracted R. batava males and females. Olfactory cues have a large impact on insect behaviour and fitness consequently showing potential in pest management. Yeast released volatiles are used by insects as olfactory cues for finding feeding and oviposition sites. The yeast strain SB-16-15 was isolated from spontaneous fermentation of Hippophae rhamnoides berries and identified as Pichia kudriavzevii. Thirty-nine volatiles were sampled from the headspace of P. kudriavzevii yeasts by solid phase micro extraction and identified by gas chromatography and mass spectrometry techniques. Ten of those volatiles elicited antennal responses of Rhagoletis batava flies, one of the most serious pest of H. rhamnoides berries. In the two-choice experiments, R. batava flies preferred the mixture composed of nine synthetic compounds analogous to electroanntenographic active volatiles released by the yeasts compare to the solvent control. Female flies were significantly attracted to the mixture at the concentration 0.1 µL mL−1 and showed no preference to the mixture at the concentration 1 µL mL−1 versus control while males reacted positively to the synthetic blend at the concentration 1 µL mL−1. Herein, for the first time, behaviour modifying effect of H. rhamnoides berry related yeast volatiles was shown suggesting these semiochemicals have potential in use for monitoring R. batava flies.